PMID- 24374786
OWN - NLM
STAT- MEDLINE
DCOM- 20150105
LR  - 20211021
IS  - 1590-3478 (Electronic)
IS  - 1590-1874 (Linking)
VI  - 35
IP  - 6
DP  - 2014 Jun
TI  - Activation of NMDA receptor of glutamate influences MMP-2 activity and 
      proliferation of glioma cells.
PG  - 823-9
LID - 10.1007/s10072-013-1604-5 [doi]
AB  - Glioblastoma multiforme (GBM) is the most common malignant glioma, which has high 
      proliferative rate and an extremely invasive phenotype. Major limitations in the 
      effective treatment of malignant gliomas are the proliferation and infiltration 
      into the surrounding brain tissue. Although studies have shown that various 
      stimuli promote glioma cell proliferation and invasion, the underlying mechanisms 
      remain largely unknown. Glioma cells secrete significant amount of glutamate into 
      surrounding tissue and intracellular signaling is thought to be initiated upon 
      glutamate-induced modulation of the ion channels in GBM cells. The objective of 
      the study was to investigate the effect of activation of NMDA 
      (N-methyl-D-aspartate) receptors of glutamate on gelatinase subfamily MMPs and on 
      proliferation of glioma cells. U251MG and U87MG cell lines were maintained in 
      Dulbecco's Modified Eagle's Medium. Proliferation assay was investigated by 
      3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole 
      (MTT) assay. Matrix metalloproteinase (MMP)-2 and MMP-9 activity was investigated 
      by gelatin zymography assay. We demonstrate that activated NMDA receptors (NMDAR) 
      increased the activity of MMP-2 only in U251MG glioma cells at concentrations of 
      100 and 200 muM and increased the proliferation of both U87MG and U251MG glioma 
      cells at concentrations of 50, 100, 150 and 200 muM. Inhibition of NMDAR using 
      MK-801, a non-competitive antagonist of the NMDAR, significantly inhibited the 
      effect of activation of NMDAR on MMP-2 activity and on proliferation. We conclude 
      that NMDA receptor activation has role in activity of MMP-2 and proliferation of 
      glioma cells.
FAU - Ramaswamy, Palaniswamy
AU  - Ramaswamy P
AD  - Department of Neurochemistry, National Institute of Mental Health and 
      NeuroSciences (NIMHANS), Bangalore, Karnataka, 560029, India.
FAU - Aditi Devi, N
AU  - Aditi Devi N
FAU - Hurmath Fathima, K
AU  - Hurmath Fathima K
FAU - Dalavaikodihalli Nanjaiah, Nandakumar
AU  - Dalavaikodihalli Nanjaiah N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131229
PL  - Italy
TA  - Neurol Sci
JT  - Neurological sciences : official journal of the Italian Neurological Society and 
      of the Italian Society of Clinical Neurophysiology
JID - 100959175
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Brain Neoplasms/*metabolism
MH  - Cell Proliferation
MH  - Glioblastoma/*metabolism
MH  - Humans
MH  - Matrix Metalloproteinase 2/*metabolism
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 2014/01/01 06:00
MHDA- 2015/01/06 06:00
CRDT- 2013/12/31 06:00
PHST- 2013/08/05 00:00 [received]
PHST- 2013/12/10 00:00 [accepted]
PHST- 2013/12/31 06:00 [entrez]
PHST- 2014/01/01 06:00 [pubmed]
PHST- 2015/01/06 06:00 [medline]
AID - 10.1007/s10072-013-1604-5 [doi]
PST - ppublish
SO  - Neurol Sci. 2014 Jun;35(6):823-9. doi: 10.1007/s10072-013-1604-5. Epub 2013 Dec 
      29.