PMID- 24375479
OWN - NLM
STAT- MEDLINE
DCOM- 20140527
LR  - 20191210
IS  - 1552-8618 (Electronic)
IS  - 0730-7268 (Linking)
VI  - 33
IP  - 4
DP  - 2014 Apr
TI  - An inter-laboratory study on the variability in measured concentrations of 
      17beta-estradiol, testosterone, and 11-ketotestosterone in white sucker: 
      implications and recommendations.
PG  - 847-57
LID - 10.1002/etc.2503 [doi]
AB  - Endocrine-disrupting chemicals are exogenous substances that can impact the 
      reproduction of fish, potentially by altering circulating concentrations of 
      17beta-estradiol (E2), testosterone (T), and 11-ketotestosterone (11-KT). Common 
      methods to measure steroids in plasma samples include radioimmunoassays (RIAs) 
      and enzyme-linked immunosorbant assays (ELISAs). The present study examines 
      variability in E2, T, and 11-KT across 8 laboratories measuring reference and 
      pulp mill effluent-exposed white sucker (Catostomus commersoni) plasma. We 
      examine the contribution of assay type (RIA vs ELISA), standardized hormone 
      extraction, location of values on the standard curve (upper and lower limits), 
      and other variables on the ability to distinguish hormone levels between 
      reference and exposed fish and the impact of these variables on quantitation of 
      hormones in different laboratories. Of the 8 participating laboratories, 7 of 8 
      and 7 of 7 identified differences between sites for female E2 and female T, 
      respectively, and 7 of 7 and 4 of 5 identified no differences between male T and 
      male 11-KT. Notably, however, the ng/mL concentration of steroids measured across 
      laboratories varied by factors of 10-, 6-, 14-, and 10-fold, respectively. Within 
      laboratory intra-assay variability was generally acceptable and below 15%. 
      Factors contributing to interlaboratory variability included calculation errors, 
      assay type, and methodology. Based on the interlaboratory variability detected, 
      we provide guidelines and recommendations to improve the accuracy and precision 
      of steroid measurements in fish ecotoxicology studies.
CI  - (c) 2013 SETAC.
FAU - Feswick, April
AU  - Feswick A
AD  - Department of Biology, University of New Brunswick, Saint John, New Brunswick, 
      Canada.
FAU - Ankley, Gerald T
AU  - Ankley GT
FAU - Denslow, Nancy
AU  - Denslow N
FAU - Ellestad, Laura E
AU  - Ellestad LE
FAU - Fuzzen, Meghan
AU  - Fuzzen M
FAU - Jensen, Kathleen M
AU  - Jensen KM
FAU - Kroll, Kevin
AU  - Kroll K
FAU - Lister, Andrea
AU  - Lister A
FAU - MacLatchy, Deborah L
AU  - MacLatchy DL
FAU - McMaster, Mark E
AU  - McMaster ME
FAU - Orlando, Edward F
AU  - Orlando EF
FAU - Servos, Mark R
AU  - Servos MR
FAU - Tetreault, Gerald R
AU  - Tetreault GR
FAU - Van Den Heuvel, Michael R
AU  - Van Den Heuvel MR
FAU - Munkittrick, Kelly R
AU  - Munkittrick KR
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140225
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 3XMK78S47O (Testosterone)
RN  - 4TI98Z838E (Estradiol)
RN  - KF38W1A85U (11-ketotestosterone)
SB  - IM
MH  - Animals
MH  - Cypriniformes/*blood
MH  - Environmental Monitoring
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Estradiol/*blood
MH  - Female
MH  - Male
MH  - Radioimmunoassay
MH  - Reproducibility of Results
MH  - Testosterone/*analogs & derivatives/*blood
OTO - NOTNLM
OT  - Androgens, Estrogenic compounds
OT  - Endocrine disruptors
OT  - Enzyme-linked immunosorbant assays
OT  - Interlaboratory
OT  - Radioimmunoassay
EDAT- 2014/01/01 06:00
MHDA- 2014/05/28 06:00
CRDT- 2013/12/31 06:00
PHST- 2013/08/01 00:00 [received]
PHST- 2013/09/16 00:00 [revised]
PHST- 2013/12/03 00:00 [accepted]
PHST- 2013/12/31 06:00 [entrez]
PHST- 2014/01/01 06:00 [pubmed]
PHST- 2014/05/28 06:00 [medline]
AID - 10.1002/etc.2503 [doi]
PST - ppublish
SO  - Environ Toxicol Chem. 2014 Apr;33(4):847-57. doi: 10.1002/etc.2503. Epub 2014 Feb 
      25.