PMID- 24376261
OWN - NLM
STAT- MEDLINE
DCOM- 20150312
LR  - 20220330
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 42
IP  - 8
DP  - 2014 Aug
TI  - Assessment of Her-2/neu status using immunocytochemistry and fluorescence in situ 
      hybridization on fine-needle aspiration cytology smears: experience from a 
      tertiary care centre in India.
PG  - 726-31
LID - 10.1002/dc.23088 [doi]
AB  - Breast carcinoma shows amplification/overexpression of Her-2/neu in  approximately 20-30% of 
      cases. The determination of Her-2/neu expression accurately is vital in clinical 
      practice as it has significant predictive value and eligibility for anti 
      Her-2/neu therapy. Amplification and overexpression of Her-2/neu gene is 
      traditionally identified by fluorescence in situ hybridization (FISH) and 
      immunohistochemistry (IHC) on tissue sections; only a few studies have evaluated 
      feasibility of these techniques on cytological smears. One hundred cases of 
      breast cancer with fine-needle aspiration cytology (FNAC) samples and 
      corresponding surgically resected specimen were selected. Immunocytochemistry 
      (ICC) and FISH for Her-2/neu was done on FNA smears, whereas IHC was performed on 
      corresponding tissue sections. Diagnostic accuracy of ICC was 99% when compared 
      with IHC. Comparison of FISH results with IHC showed 100% concordance. Unlike 
      many centers in West, FNAC is still routinely performed in developing countries 
      like India where vast majority of breast cancer cases present as palpable lumps. 
      The high rates of accuracy of ICC and FISH for Her-2/neu detection can make FNAC 
      a relevant first line of investigation as a cost effective model with a rapid 
      turn-around time, providing complete information necessary for initial management 
      of breast cancer patients.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Durgapal, Prashant
AU  - Durgapal P
AD  - Department of Pathology, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Mathur, Sandeep R
AU  - Mathur SR
FAU - Kalamuddin, Md
AU  - Kalamuddin M
FAU - Datta Gupta, Siddhartha
AU  - Datta Gupta S
FAU - Parshad, Rajinder
AU  - Parshad R
FAU - Julka, Pramod K
AU  - Julka PK
FAU - Panda, S K
AU  - Panda SK
LA  - eng
PT  - Journal Article
DEP - 20131220
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Fine-Needle
MH  - Breast Neoplasms/*metabolism/*pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - India
MH  - Middle Aged
MH  - Receptor, ErbB-2/*metabolism
MH  - *Tertiary Care Centers
OTO - NOTNLM
OT  - FISH
OT  - FNAC
OT  - breast cancer
OT  - immunocytochemistry
EDAT- 2014/01/01 06:00
MHDA- 2015/03/13 06:00
CRDT- 2013/12/31 06:00
PHST- 2013/06/04 00:00 [received]
PHST- 2013/11/04 00:00 [revised]
PHST- 2013/12/03 00:00 [accepted]
PHST- 2013/12/31 06:00 [entrez]
PHST- 2014/01/01 06:00 [pubmed]
PHST- 2015/03/13 06:00 [medline]
AID - 10.1002/dc.23088 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2014 Aug;42(8):726-31. doi: 10.1002/dc.23088. Epub 2013 Dec 20.