PMID- 24377422
OWN - NLM
STAT- MEDLINE
DCOM- 20140722
LR  - 20140320
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 62
IP  - 4
DP  - 2014 Jan 29
TI  - Novel highly active recombinant glutaredoxin from Chlorella sorokiniana T-89.
PG  - 927-33
LID - 10.1021/jf405213h [doi]
AB  - Glutaredoxin (Grx) is a thiol/disulfide oxidoreductase that maintains the 
      cellular thiol/disulfide ratio. A 321 bp cDNA fragment encoding a putative Grx 
      (named CsT-89Grx) was cloned from heat-tolerant Chlorella sorokiniana T-89 and 
      expressed in an Escherichia coli system. The sequence analysis of CsT-89Grx and 
      site-directed mutations showed that the putative active site within the CPYC 
      motif belonged to the dithiol superfamily. The biochemical property analyses 
      showed that the optimal pH and temperature of CsT-89Grx are pH 8.5 and 50  degrees C, 
      respectively. The activity of CsT-89Grx showed high thermal stability (retained 
      70% activity at 80  degrees C for 30 min) and broad pH stability (retained over 70% 
      activity for 1 h) ranging from pH 3 to 11. The kinetic parameter kcat/Km was 
      20,982 min(-1) mM(-1), which suggested that CsT-89Grx exhibited the highest 
      catalytic efficiency in reducing the disulfide bond among all the Grx reported in 
      the related literature and is therefore potentially useful for industrial 
      applications.
FAU - Chuang, Hsu-Han
AU  - Chuang HH
AD  - Department of Food Science and Biotechnology, National Chung Hsing University , 
      Taichung, Taiwan.
FAU - Cheng, Chu-Ying
AU  - Cheng CY
FAU - Chen, Yu-Ting
AU  - Chen YT
FAU - Shaw, Jei-Fu
AU  - Shaw JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140114
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (DNA, Complementary)
RN  - 0 (Glutaredoxins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Catalytic Domain
MH  - Chlorella/*enzymology/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - Enzyme Stability
MH  - Escherichia coli
MH  - Gene Expression
MH  - Glutaredoxins/chemistry/*genetics/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Infrared Rays
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Recombinant Proteins
MH  - Sequence Alignment
EDAT- 2014/01/01 06:00
MHDA- 2014/07/23 06:00
CRDT- 2014/01/01 06:00
PHST- 2014/01/01 06:00 [entrez]
PHST- 2014/01/01 06:00 [pubmed]
PHST- 2014/07/23 06:00 [medline]
AID - 10.1021/jf405213h [doi]
PST - ppublish
SO  - J Agric Food Chem. 2014 Jan 29;62(4):927-33. doi: 10.1021/jf405213h. Epub 2014 
      Jan 14.