PMID- 24378313 OWN - NLM STAT- MEDLINE DCOM- 20140929 LR - 20220316 IS - 1872-6283 (Electronic) IS - 0379-0738 (Linking) VI - 234 DP - 2014 Jan TI - Development and validation of a single LC-MS/MS assay following SPE for simultaneous hair analysis of amphetamines, opiates, cocaine and metabolites. PG - 132-8 LID - S0379-0738(13)00490-8 [pii] LID - 10.1016/j.forsciint.2013.11.004 [doi] AB - The two major challenges in hair analysis are the limited amount of samples usually available and the low targeted concentrations. To overcome these limitations, a liquid chromatography-electrospray-tandem mass spectrometry method (LC-ESI-MS/MS) allowing the simultaneous analysis of 17 amphetamines (amphetamine, BDB, m-CPP, dexfenfluramine, DOB, DOM, ephedrine, MBDB, MDA, MDEA, MDMA, methamphetamine, methylphenidate, 4-MTA, norephedrine, norfenfluramine and PMA), 5 opiates (morphine, codeine, heroin, ethylmorphine, and 6AM), cocaine and 5 metabolites [ecgonine methyl ester (EME), benzoylecgonine (BZE), anhydroecgonine methyl ester (AME), cocaethylene, and norcocaine] has been developed. The validation procedure included linearity, intra-day and inter-day variability and accuracy for 5 days (5 replicates at 3 concentration levels). Proficiency studies were used to check the accuracy of the method. As a result, all amphetamines, opiates and cocaine derivatives were satisfactory identified by 2 MRM transitions in 15 min. Calibration curves were performed by a quadratic 1/X weighted regression. The calibration model fits from 0.05 to 10 ng/mg. The limits of detection (LODs) range between 0.005 and 0.030 ng/mg. Precision has been checked by intra-day and inter-day RSD, and associated relative bias, which were lower than 25% for the limits of quantifications (LOQs) and lower than 20% for the other levels tested. This method was routinely applied to hair samples: two positive results of adult drug addicts are presented. CI - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved. FAU - Imbert, L AU - Imbert L AD - Department of Pharmacology and Toxicology, University Hospital, 2, Avenue Martin-Luther-King, 87042 Limoges Cedex, France. Electronic address: laurent.imbert@unilim.fr. FAU - Dulaurent, S AU - Dulaurent S AD - Department of Pharmacology and Toxicology, University Hospital, 2, Avenue Martin-Luther-King, 87042 Limoges Cedex, France. Electronic address: sylvain.dulaurent@unilim.fr. FAU - Mercerolle, M AU - Mercerolle M AD - Department of Pharmacology and Toxicology, University Hospital, 2, Avenue Martin-Luther-King, 87042 Limoges Cedex, France. Electronic address: mm@shimadzu.fr. FAU - Morichon, J AU - Morichon J AD - Department of Pharmacology and Toxicology, University Hospital, 2, Avenue Martin-Luther-King, 87042 Limoges Cedex, France. Electronic address: CG@chu-limoges.fr. FAU - Lachatre, G AU - Lachatre G AD - Department of Pharmacology and Toxicology, University Hospital, 2, Avenue Martin-Luther-King, 87042 Limoges Cedex, France; Laboratory of Toxicology, Faculty of Pharmacy, 2 rue Docteur Raymond Marcland, 87000 Limoges, France. Electronic address: gerard.lachatre@unilim.fr. FAU - Gaulier, J-M AU - Gaulier JM AD - Department of Pharmacology and Toxicology, University Hospital, 2, Avenue Martin-Luther-King, 87042 Limoges Cedex, France. Electronic address: jm-gaulier@unilim.fr. LA - eng PT - Journal Article PT - Validation Study DEP - 20131115 PL - Ireland TA - Forensic Sci Int JT - Forensic science international JID - 7902034 RN - 0 (Amphetamines) RN - 0 (Analgesics, Opioid) RN - 0 (Narcotics) RN - 3SL7BR2M1E (norcocaine) RN - 43021-26-7 (anhydroecgonine methyl ester) RN - 5353I8I6YS (benzoylecgonine) RN - FJO3071W5Y (cocaethylene) RN - I5Y540LHVR (Cocaine) RN - Y35FJB3QBJ (ecgonine methyl ester) SB - IM MH - Amphetamines/*analysis MH - Analgesics, Opioid/*analysis MH - Chromatography, Liquid MH - Cocaine/analogs & derivatives/*analysis MH - Forensic Toxicology/methods MH - Hair/*chemistry MH - Humans MH - Limit of Detection MH - Male MH - Narcotics/*analysis MH - Spectrometry, Mass, Electrospray Ionization MH - Substance Abuse Detection/methods MH - Tandem Mass Spectrometry OTO - NOTNLM OT - Amphetamines OT - Cocaine OT - Forensic toxicology OT - Hair OT - Opiates OT - Tandem mass spectrometry EDAT- 2014/01/01 06:00 MHDA- 2014/09/30 06:00 CRDT- 2014/01/01 06:00 PHST- 2013/08/12 00:00 [received] PHST- 2013/10/28 00:00 [revised] PHST- 2013/11/06 00:00 [accepted] PHST- 2014/01/01 06:00 [entrez] PHST- 2014/01/01 06:00 [pubmed] PHST- 2014/09/30 06:00 [medline] AID - S0379-0738(13)00490-8 [pii] AID - 10.1016/j.forsciint.2013.11.004 [doi] PST - ppublish SO - Forensic Sci Int. 2014 Jan;234:132-8. doi: 10.1016/j.forsciint.2013.11.004. Epub 2013 Nov 15.