PMID- 24378336
OWN - NLM
STAT- MEDLINE
DCOM- 20140326
LR  - 20211021
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 386
IP  - 2
DP  - 2014 Feb 15
TI  - BDNF and NT4 play interchangeable roles in gustatory development.
PG  - 308-20
LID - S0012-1606(13)00693-3 [pii]
LID - 10.1016/j.ydbio.2013.12.031 [doi]
AB  - A limited number of growth factors are capable of regulating numerous 
      developmental processes, but how they accomplish this is unclear. The gustatory 
      system is ideal for examining this issue because the neurotrophins brain-derived 
      neurotrophic factor (BDNF) and neurotrophin-4 (NT4) have different developmental 
      roles although both of them activate the same receptors, TrkB and p75. Here we 
      first investigated whether the different roles of BDNF and NT4 are due to their 
      differences in temporal and spatial expression patterns. Then, we asked whether 
      or not these two neurotrophins exert their unique roles on the gustatory system 
      by regulating different sets of downstream genes. By using Bdnf(Nt4/Nt4) mice, in 
      which the coding region for BDNF is replaced with NT4, we examined whether the 
      different functions of BDNF and NT4 are interchangeable during taste development. 
      Our results demonstrated that NT4 could mediate most of the unique roles of BDNF 
      during taste development. Specifically, caspase-3-mediated cell death, which was 
      increased in the geniculate ganglion in Bdnf(-/-) mice, was rescued in 
      Bdnf(Nt4/Nt4) mice. In BDNF knockout mice, tongue innervation was disrupted, and 
      gustatory axons failed to reach their targets. However, disrupted innervation was 
      rescued and target innervation is normal when NT4 replaced BDNF. Genome wide 
      expression analyses revealed that BDNF and NT4 mutant mice exhibited different 
      gene expression profiles in the gustatory (geniculate) ganglion. Compared to wild 
      type, the expression of differentiation-, apoptosis- and axon guidance-related 
      genes was changed in BDNF mutant mice, which is consistent with their different 
      roles during taste development. However, replacement of BDNF by NT4 rescued these 
      gene expression changes. These findings indicate that the functions of BDNF and 
      NT4 in taste development are interchangeable. Spatial and temporal differences in 
      BDNF and NT4 expression can regulate differential gene expression in vivo and 
      determine their specific roles during development.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Huang, Tao
AU  - Huang T
AD  - Department of Anatomical Sciences and Neurobiology, University of Louisville 
      School of Medicine, Louisville, KY 40292, USA.
FAU - Krimm, Robin F
AU  - Krimm RF
AD  - Department of Anatomical Sciences and Neurobiology, University of Louisville 
      School of Medicine, Louisville, KY 40292, USA. Electronic address: 
      rfkrim01@louisville.edu.
LA  - eng
GR  - R01 DC007176/DC/NIDCD NIH HHS/United States
GR  - R01 DC009418/DC/NIDCD NIH HHS/United States
GR  - DC007176/DC/NIDCD NIH HHS/United States
GR  - DC009418/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20131227
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (1,1'-dioleyl-3,3,3',3'-tetramethylindocarbocyanine)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carbocyanines)
RN  - 0 (Nerve Growth Factors)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Carbocyanines
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental/genetics/*physiology
MH  - Geniculate Ganglion/metabolism
MH  - Immunohistochemistry
MH  - Laser Capture Microdissection
MH  - Mice
MH  - Mice, Knockout
MH  - Microarray Analysis
MH  - Nerve Growth Factors/genetics/*metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Taste/*physiology
PMC - PMC3950349
MID - NIHMS558366
OTO - NOTNLM
OT  - Brain derived neurotrophic factor
OT  - Geniculate ganglion neurons
OT  - Neurotrophin-4
OT  - Neurotrophins
OT  - Taste
EDAT- 2014/01/01 06:00
MHDA- 2014/03/29 06:00
PMCR- 2015/02/15
CRDT- 2014/01/01 06:00
PHST- 2013/10/02 00:00 [received]
PHST- 2013/12/17 00:00 [revised]
PHST- 2013/12/20 00:00 [accepted]
PHST- 2014/01/01 06:00 [entrez]
PHST- 2014/01/01 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
PHST- 2015/02/15 00:00 [pmc-release]
AID - S0012-1606(13)00693-3 [pii]
AID - 10.1016/j.ydbio.2013.12.031 [doi]
PST - ppublish
SO  - Dev Biol. 2014 Feb 15;386(2):308-20. doi: 10.1016/j.ydbio.2013.12.031. Epub 2013 
      Dec 27.