PMID- 24378591
OWN - NLM
STAT- MEDLINE
DCOM- 20150205
LR  - 20211021
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 15
IP  - 2
DP  - 2014
TI  - Recombinant Kluyveromyces lactis expressing highly pathogenic porcine 
      reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated 
      immune responses in mice.
PG  - 199-208
AB  - Currently, killed-virus and modified-live porcine reproductive and respiratory 
      syndrome virus (PRRSV) vaccines are used to control porcine reproductive and 
      respiratory syndrome. However, both types of vaccines have inherent drawbacks; 
      accordingly, the development of novel PRRSV vaccines is urgently needed. Previous 
      studies have suggested that yeast possesses adjuvant activities, and it has been 
      used as an expression vehicle to elicit immune responses to foreign antigens. In 
      this report, recombinant Kluyveromyces lactis expressing GP5 of HP-PRRSV 
      (Yeast-GP5) was generated and immune responses to this construct were analyzed in 
      mice. Intestinal mucosal PRRSV-specific sIgA antibody and higher levels of IFN-gamma 
      in spleen CD4(+) and CD8(+) T cells were induced by oral administration of 
      Yeast-GP5. Additionally, Yeast-GP5 administered subcutaneously evoked vigorous 
      cell-mediated immunity, and PRRSV-specific lymphocyte proliferation and IFN-gamma 
      secretion were detected in the splenocytes of mice. These results suggest that 
      Yeast-GP5 has the potential for use as a vaccine for PRRSV in the future.
FAU - Zhao, Haiyan
AU  - Zhao H
AD  - State Key Laboratory of Agrobiotechnology, Key Laboratory of Soil Microbiology, 
      Department of Microbiology and Immunology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China.
FAU - Wang, Yalan
AU  - Wang Y
FAU - Ma, Zhitao
AU  - Ma Z
FAU - Wang, Yongqiang
AU  - Wang Y
FAU - Feng, Wen-hai
AU  - Feng WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131227
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Antibodies, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antibodies, Viral/*immunology
MH  - B-Lymphocytes/immunology/virology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Immunity, Cellular
MH  - *Immunity, Mucosal
MH  - Injections, Subcutaneous
MH  - Kluyveromyces/genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Recombinant Proteins/genetics/immunology
MH  - T-Lymphocytes/immunology/virology
MH  - Viral Envelope Proteins/*genetics/*immunology
MH  - Viral Vaccines/administration & dosage/*pharmacology
PMC - PMC4087221
OTO - NOTNLM
OT  - GP5
OT  - Kluyveromyces lactis yeast
OT  - PRRSV
OT  - immune responses
OT  - vaccine
EDAT- 2014/01/01 06:00
MHDA- 2015/02/06 06:00
PMCR- 2014/06/01
CRDT- 2014/01/01 06:00
PHST- 2013/09/10 00:00 [received]
PHST- 2013/11/23 00:00 [accepted]
PHST- 2014/01/01 06:00 [entrez]
PHST- 2014/01/01 06:00 [pubmed]
PHST- 2015/02/06 06:00 [medline]
PHST- 2014/06/01 00:00 [pmc-release]
AID - jvs.2013.093 [pii]
AID - 10.4142/jvs.2014.15.2.199 [doi]
PST - ppublish
SO  - J Vet Sci. 2014;15(2):199-208. doi: 10.4142/jvs.2014.15.2.199. Epub 2013 Dec 27.