PMID- 24378594
OWN - NLM
STAT- MEDLINE
DCOM- 20141028
LR  - 20140224
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 37
IP  - 1
DP  - 2014 Jan
TI  - Mild maternal iron deficiency anemia induces DPOAE suppression and cochlear hair 
      cell apoptosis by caspase activation in young guinea pigs.
PG  - 291-9
LID - S1382-6689(13)00265-2 [pii]
LID - 10.1016/j.etap.2013.11.024 [doi]
AB  - Iron deficiency (ID) anemia (IDA) alters auditory neural normal development in 
      the mammalian cochlea. Previous results suggest that mild maternal IDA during 
      pregnancy and lactation altered the hearing and nervous system development of the 
      young offspring, but the mechanisms underlying the association are incompletely 
      understood. The objective of this study was to evaluate the role of apoptosis in 
      the development of sensory hair cells following mild maternal IDA during 
      pregnancy and lactation. We established a maternal anemia model in female guinea 
      pigs by using a mild iron deficient diet. The offspring were weaned on postnatal 
      day (PND) 9 and then was given the iron sufficient diet. Maternal blood samples 
      were collected on gestational day (GD) 21, GD 42, GD 63 and PND 9, serum level of 
      iron (SI) or hemoglobin (Hb) was measured. Blood samples of pups were collected 
      on PND 9 for SI measurement. On PND 24, pups were examined the distortion product 
      otoacoustic emission (DPOAE) task, and then the cochleae were harvested for 
      assessment of apoptosis by immunohistochemistry of cysteine-aspartic acid 
      proteases 3/9 (caspase-3/9) and terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end-labeling (TUNEL) assay, and by double immunofluorescence for the 
      colocalization of TUNEL and caspase-3. Blood samples of pups were collected on 
      PND 24 for SI and Hb measurements. Here we show that mild maternal IDA during 
      pregnancy and lactation resulted in hearing impairment, decreased hair cell 
      number, caspase-3/9 activation and increased apoptotic cell number of young 
      guinea pigs. These results indicate a key role for apoptosis in inhibition of 
      hair cell development, caused by mild maternal IDA during pregnancy and 
      lactation.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Yu, Fei
AU  - Yu F
AD  - Department of Nutrition and Food Hygiene, School of Public Health, China Medical 
      University, 92 North 2nd Road, Heping District, Shenyang, China. Electronic 
      address: yufei@mail.cmu.edu.cn.
FAU - Hao, Shuai
AU  - Hao S
AD  - Department of Otolaryngology, First Affiliated Hospital of China Medical 
      University, Shenyang, China.
FAU - Zhao, Yue
AU  - Zhao Y
AD  - Department of Nutrition and Food Hygiene, School of Public Health, China Medical 
      University, 92 North 2nd Road, Heping District, Shenyang, China.
FAU - Ren, Yahao
AU  - Ren Y
AD  - Department of Nutrition and Food Hygiene, School of Public Health, China Medical 
      University, 92 North 2nd Road, Heping District, Shenyang, China.
FAU - Yang, Jun
AU  - Yang J
AD  - Department of Nutrition and Food Hygiene, School of Public Health, China Medical 
      University, 92 North 2nd Road, Heping District, Shenyang, China.
FAU - Sun, Xiance
AU  - Sun X
AD  - Department of Occupational and Environmental of Health, Dalian Medical 
      University, No. 9 Western Section of Lushun South Road, Dalian, Liaoning, China. 
      Electronic address: sunxiance@gmail.com.
FAU - Chen, Jie
AU  - Chen J
AD  - Department of Occupational and Environmental of Health, School of Public Health, 
      China Medical University, Shenyang, China. Electronic address: feigo_dl@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131209
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
RN  - 0 (Hemoglobins)
RN  - E1UOL152H7 (Iron)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 9)
SB  - IM
MH  - Acoustic Stimulation
MH  - Anemia, Iron-Deficiency/blood/*complications/metabolism/physiopathology
MH  - Animals
MH  - Apoptosis
MH  - Caspase 3/*metabolism
MH  - Caspase 9/*metabolism
MH  - DNA Fragmentation
MH  - Female
MH  - Guinea Pigs
MH  - Hair Cells, Auditory/*metabolism
MH  - Hearing Loss/*etiology/metabolism/physiopathology
MH  - Hemoglobins/analysis
MH  - Iron/blood
MH  - Lactation/physiology
MH  - Pregnancy
OTO - NOTNLM
OT  - Apoptosis
OT  - Iron deficiency anemia
OT  - Ototoxicity
EDAT- 2014/01/01 06:00
MHDA- 2014/10/29 06:00
CRDT- 2014/01/01 06:00
PHST- 2013/10/12 00:00 [received]
PHST- 2013/11/22 00:00 [revised]
PHST- 2013/11/28 00:00 [accepted]
PHST- 2014/01/01 06:00 [entrez]
PHST- 2014/01/01 06:00 [pubmed]
PHST- 2014/10/29 06:00 [medline]
AID - S1382-6689(13)00265-2 [pii]
AID - 10.1016/j.etap.2013.11.024 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2014 Jan;37(1):291-9. doi: 10.1016/j.etap.2013.11.024. 
      Epub 2013 Dec 9.