PMID- 24380400
OWN - NLM
STAT- MEDLINE
DCOM- 20160125
LR  - 20150507
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 24
IP  - 5
DP  - 2015
TI  - Two distinct mechanisms mediate the involvement of bone marrow cells in islet 
      remodeling: neogenesis of insulin-producing cells and support of islet recovery.
PG  - 879-90
LID - 10.3727/096368913X676899 [doi]
AB  - We have recently reported that small-sized bone marrow cells (BMCs) isolated by 
      counterflow centrifugal elutriation and depleted of lineage markers (Fr25lin(-)) 
      have the capacity to differentiate and contribute to regeneration of injured 
      islets. In this study, we assess some of the characteristics of these cells 
      compared to elutriated hematopoietic progenitors (R/O) and whole BMCs in a murine 
      model of streptozotocin-induced chemical diabetes. The GFP(bright)CD45(+) progeny 
      of whole BMCs and R/O progenitors progressively infiltrate the pancreas with 
      evolution of donor chimerism; are found at islet perimeter, vascular, and ductal 
      walls; and have a modest impact on islet recovery from injury. In contrast, 
      Fr25lin(-) cells incorporate in the islets, convert to GFP(dim)CD45(-)PDX-1(+) 
      phenotypes, produce proinsulin, and secrete insulin with significant contribution 
      to stabilization of glucose homeostasis. The elutriated Fr25lin(-) cells express 
      low levels of CD45 and are negative for SCA-1 and c-kit, as removal of cells 
      expressing these markers did not impair conversion to produce insulin. BMCs 
      mediate two synergistic mechanisms that contribute to islet recovery from injury: 
      support of islet remodeling by hematopoietic cells and neogenesis of 
      insulin-producing cells from stem cells.
FAU - Iskovich, Svetlana
AU  - Iskovich S
AD  - Frankel Laboratory, Schneider Children's Medical Center of Israel, Petach Tikva, 
      Israel.
FAU - Goldenberg-Cohen, Nitza
AU  - Goldenberg-Cohen N
FAU - Sadikov, Tamila
AU  - Sadikov T
FAU - Yaniv, Isaac
AU  - Yaniv I
FAU - Stein, Jerry
AU  - Stein J
FAU - Askenasy, Nadir
AU  - Askenasy N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131230
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*metabolism
MH  - *Bone Marrow Transplantation
MH  - *Cell Differentiation
MH  - Diabetes Mellitus, Experimental/metabolism/*therapy
MH  - Insulin-Secreting Cells/*metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Transplantation Chimera/*metabolism
EDAT- 2014/01/02 06:00
MHDA- 2016/01/26 06:00
CRDT- 2014/01/02 06:00
PHST- 2014/01/02 06:00 [entrez]
PHST- 2014/01/02 06:00 [pubmed]
PHST- 2016/01/26 06:00 [medline]
AID - content-ct1082Iskovich [pii]
AID - 10.3727/096368913X676899 [doi]
PST - ppublish
SO  - Cell Transplant. 2015;24(5):879-90. doi: 10.3727/096368913X676899. Epub 2013 Dec 
      30.