PMID- 24380861
OWN - NLM
STAT- MEDLINE
DCOM- 20140414
LR  - 20140211
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 443
IP  - 4
DP  - 2014 Jan 24
TI  - Molecular cloning, functional characterization and antiviral activity of porcine 
      DDX3X.
PG  - 1169-75
LID - S0006-291X(13)02169-4 [pii]
LID - 10.1016/j.bbrc.2013.12.098 [doi]
AB  - Human DDX3X is a newly discovered DEAD-box RNA helicase. In addition to 
      involvement of eukaryotic gene expression regulation, human DDX3X has recently 
      been demonstrated to be a critical molecule in innate immune signaling pathways 
      and to contribute to type I interferon (IFN) induction. In the present study, 
      porcine DDX3X was cloned by RT-PCR from PK-15 cells and its function in 
      regulating IFN-beta was characterized. The putative porcine DDX3X ORF encodes 662 
      amino acids possessing several conserved motifs. Sequence alignments indicated 
      that porcine DDX3X has high identity at the amino acid level to those of horse 
      (96.7%), mouse (97.6%), cattle (98.5%), dog (98.6%) and human (98.9%). Ectopic 
      expression of porcine DDX3X significantly activated IFN-beta expression, whereas 
      knockdown of porcine DDX3X inhibited dsRNA- or Sendai virus (SeV)-induced IFN-beta. 
      Furthermore, porcine DDX3X co-localized with IPS-1, TBK1 and IKKepsilon, and enhanced 
      IFN-beta promoter activation induced by these molecules. We also investigated the 
      role of porcine DDX3X during porcine reproductive and respiratory syndrome virus 
      (PRRSV) infection and found that overexpression of DDX3X significantly inhibited 
      PRRSV replication, indicating that DDX3X is a potential antiviral agent.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Chen, Quangang
AU  - Chen Q
AD  - Division of Animal Infectious Diseases, State Key Laboratory of Agricultural 
      Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 
      Wuhan 430070, China.
FAU - Liu, Qin
AU  - Liu Q
AD  - Division of Animal Infectious Diseases, State Key Laboratory of Agricultural 
      Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 
      Wuhan 430070, China.
FAU - Liu, Dezhi
AU  - Liu D
AD  - Division of Animal Infectious Diseases, State Key Laboratory of Agricultural 
      Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 
      Wuhan 430070, China.
FAU - Wang, Dang
AU  - Wang D
AD  - Division of Animal Infectious Diseases, State Key Laboratory of Agricultural 
      Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 
      Wuhan 430070, China.
FAU - Chen, Huanchun
AU  - Chen H
AD  - Division of Animal Infectious Diseases, State Key Laboratory of Agricultural 
      Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 
      Wuhan 430070, China.
FAU - Xiao, Shaobo
AU  - Xiao S
AD  - Division of Animal Infectious Diseases, State Key Laboratory of Agricultural 
      Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 
      Wuhan 430070, China.
FAU - Fang, Liurong
AU  - Fang L
AD  - Division of Animal Infectious Diseases, State Key Laboratory of Agricultural 
      Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 
      Wuhan 430070, China. Electronic address: fangxwsb@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131228
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antiviral Agents)
RN  - 0 (RNA, Messenger)
RN  - 77238-31-4 (Interferon-beta)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antiviral Agents/metabolism
MH  - Cattle
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DEAD-box RNA Helicases/*genetics/immunology/*physiology
MH  - Dogs
MH  - Humans
MH  - I-kappa B Kinase/metabolism
MH  - Immunity, Innate
MH  - Interferon-beta/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porcine respiratory and reproductive syndrome virus/physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Swine
MH  - Virus Replication
OTO - NOTNLM
OT  - Antiviral function
OT  - DDX3X
OT  - Interferon signaling
OT  - Porcine
EDAT- 2014/01/02 06:00
MHDA- 2014/04/15 06:00
CRDT- 2014/01/02 06:00
PHST- 2013/12/16 00:00 [received]
PHST- 2013/12/19 00:00 [accepted]
PHST- 2014/01/02 06:00 [entrez]
PHST- 2014/01/02 06:00 [pubmed]
PHST- 2014/04/15 06:00 [medline]
AID - S0006-291X(13)02169-4 [pii]
AID - 10.1016/j.bbrc.2013.12.098 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2014 Jan 24;443(4):1169-75. doi: 
      10.1016/j.bbrc.2013.12.098. Epub 2013 Dec 28.