PMID- 24382092 OWN - NLM STAT- MEDLINE DCOM- 20150401 LR - 20171116 IS - 1369-1619 (Electronic) IS - 0960-3123 (Linking) VI - 24 IP - 6 DP - 2014 TI - Changes in the IgE-reacting protein profiles of Acer negundo, Platanus x acerifolia and Quercus robur pollen in response to ozone treatment. PG - 515-27 LID - 10.1080/09603123.2013.865716 [doi] AB - This study aims to investigate the effects of O3 in protein content and immunoglobulin E (IgE)-binding profiles of Acer negundo, Platanus x acerifolia and Quercus robur pollen. Pollen was exposed to O3 in an environmental chamber, at half, equal and four times the limit value for the human health protection in Europe. Pollen total soluble protein was determined with Coomassie Protein Assay Reagent, and the antigenic and allergenic properties were investigated by SDS-PAGE and immunological techniques using patients' sera. O3 exposure affected total soluble protein content and some protein species within the SDS-PAGE protein profiles. Most of the sera revealed increased IgE reactivity to proteins of A. negundo and Q. robur pollen exposed to the pollutant compared with the non-exposed one, while the opposite was observed in P. x acerifolia pollen. So, the modifications seem to be species dependent, but do not necessarily imply that increase allergenicity would occur in atopic individuals. FAU - Ribeiro, Helena AU - Ribeiro H AD - a Geology Centre of University of Porto , Porto , Portugal. FAU - Duque, Laura AU - Duque L FAU - Sousa, Raquel AU - Sousa R FAU - Cruz, Ana AU - Cruz A FAU - Gomes, Carlos AU - Gomes C FAU - da Silva, Joaquim Esteves AU - da Silva JE FAU - Abreu, Ilda AU - Abreu I LA - eng PT - Journal Article DEP - 20140102 PL - England TA - Int J Environ Health Res JT - International journal of environmental health research JID - 9106628 RN - 0 (Air Pollutants) RN - 0 (Allergens) RN - 0 (Oxidants) RN - 37341-29-0 (Immunoglobulin E) RN - 66H7ZZK23N (Ozone) SB - IM MH - *Acer MH - Air Pollutants/pharmacology MH - Allergens/*drug effects/immunology MH - Immunoglobulin E/*immunology MH - Oxidants/pharmacology MH - Ozone/*pharmacology MH - Pollen/*drug effects/immunology MH - *Quercus MH - *Tracheophyta OTO - NOTNLM OT - IgE OT - allergenicity OT - ozone OT - pollen OT - soluble proteins EDAT- 2014/01/03 06:00 MHDA- 2015/04/02 06:00 CRDT- 2014/01/03 06:00 PHST- 2014/01/03 06:00 [entrez] PHST- 2014/01/03 06:00 [pubmed] PHST- 2015/04/02 06:00 [medline] AID - 10.1080/09603123.2013.865716 [doi] PST - ppublish SO - Int J Environ Health Res. 2014;24(6):515-27. doi: 10.1080/09603123.2013.865716. Epub 2014 Jan 2.