PMID- 2438330
OWN - NLM
STAT- MEDLINE
DCOM- 19870702
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 138
IP  - 11
DP  - 1987 Jun 1
TI  - Effects of ADP-ribosylation of GTP-binding protein by pertussis toxin on 
      immunoglobulin E-dependent and -independent histamine release from mast cells and 
      basophils.
PG  - 3927-34
AB  - Pretreatment of rat peritoneal mast cells, human basophils, bone marrow-derived 
      mouse mast cells (BMMC) and mouse mast cell line PT-18 cells with 1 microgram/ml 
      pertussis toxin (PT) failed to inhibit immunoglobulin E (IgE)-dependent histamine 
      release from the cells. In BMMC and PT-18 cells, even 20-hr incubation of the 
      cells with 1 microgram/ml PT, which ADP-ribosylates more than 97% of 41 kDa, 
      alpha-subunit of Ni in the cells, failed to affect the IgE-dependent release of 
      histamine or arachidonate. The results indicate that GTP-binding protein, Ni, is 
      not involved in the transduction of triggering signals induced by cross-linking 
      of IgE receptors. In contrast, pretreatment of rat mast cells with 1 ng/ml to 0.1 
      microgram/ml PT for 2 hr inhibited histamine release induced by compound 48/80 in 
      a dose-dependent manner. A similar pretreatment with PT inhibited 
      thrombin-induced histamine release from BMMC and 
      N-formyl-L-methionyl-L-leucyl-L-phenylalanine-induced histamine release from 
      human basophils in a similar dose-dependent fashion. However, even 20 hr of 
      incubation of sensitized BMMC with 1 microgram/ml PT failed to inhibit either 
      thrombin-induced or antigen-induced breakdown of phosphatidylinositides (PI), 
      i.e., the formation of inositol triphosphate and diacylglycerol, Quin-2 signal, 
      and the release of arachidonic acid. The results indicate that the inhibition of 
      thrombin-induced histamine release by PT-treatment is not due to the inhibition 
      of PI-turnover, and that Ni is not involved in thrombin-induced or 
      antigen-induced (IgE-dependent) hydrolysis of phosphatidylinositides in mast 
      cells.
FAU - Saito, H
AU  - Saito H
FAU - Okajima, F
AU  - Okajima F
FAU - Molski, T F
AU  - Molski TF
FAU - Sha'afi, R I
AU  - Sha'afi RI
FAU - Ui, M
AU  - Ui M
FAU - Ishizaka, T
AU  - Ishizaka T
LA  - eng
GR  - AI-10060/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 20762-30-5 (Adenosine Diphosphate Ribose)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 3.4.21.5 (Thrombin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Adenosine Diphosphate Ribose/*metabolism
MH  - Animals
MH  - Antigens
MH  - Basophils/*physiology
MH  - Bone Marrow Cells
MH  - Cell Membrane/metabolism
MH  - GTP-Binding Proteins/*physiology
MH  - Histamine Release/*drug effects
MH  - Humans
MH  - Immunoglobulin E/*physiology
MH  - Mast Cells/*physiology
MH  - Mice
MH  - *Pertussis Toxin
MH  - Phosphatidylinositols/metabolism
MH  - Rats
MH  - Thrombin/metabolism
MH  - Virulence Factors, Bordetella/*pharmacology
EDAT- 1987/06/01 00:00
MHDA- 1987/06/01 00:01
CRDT- 1987/06/01 00:00
PHST- 1987/06/01 00:00 [pubmed]
PHST- 1987/06/01 00:01 [medline]
PHST- 1987/06/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1987 Jun 1;138(11):3927-34.