PMID- 24383504
OWN - NLM
STAT- MEDLINE
DCOM- 20160526
LR  - 20181202
IS  - 1715-5320 (Electronic)
IS  - 1715-5312 (Linking)
VI  - 39
IP  - 1
DP  - 2014 Jan
TI  - Modulation of visceral fat adipokine secretion by dietary fatty acids and ensuing 
      changes in skeletal muscle inflammation.
PG  - 28-37
LID - 10.1139/apnm-2013-0135 [doi]
AB  - Given the link between obesity and insulin resistance, the role of 
      adipose-derived factors in communicating with skeletal muscle to affect its 
      function is important. We sought to determine if high fat diets modulate visceral 
      adipose tissue (VAT) adipokines with subsequent effects on skeletal muscle 
      inflammation and insulin sensitivity. Rats were fed (i) low fat (LF), (ii) high 
      saturated fatty acid (SFA), or (iii) high SFA with n-3 polyunsaturated fatty acid 
      (SFA/n-3 PUFA) diets for 4 weeks. VAT-derived adipokines were measured in adipose 
      conditioned medium (ACM) after 72 h. Next, skeletal muscles from LF-fed rats were 
      incubated for 8 h in (i) control buffer (CON), (ii) CON with 2 mmol.L(-1) 
      palmitate (PALM, positive control), (iii) ACM from LF, (iv) ACM from SFA, or (v) 
      ACM from SFA/n-3 PUFA. ACM from rats fed SFA and SFA/n-3 PUFA had increased (P </= 
      0.05) interleukin-6 (IL-6) (+31%) and monocyte chemoattractant protein-1 (MCP-1) 
      (+30%). Adiponectin was decreased (-29%, P </= 0.05) in ACM from SFA, and this was 
      prevented in SFA/n-3 PUFA ACM. Toll-like receptor 4 (TLR4) gene expression was 
      increased (P </= 0.05) in PALM soleus muscle (+356%) and all ACM groups 
      (+175%-191%). MCP-1 gene expression was elevated (P </= 0.05) in PALM soleus muscle 
      (+163%) and soleus muscle incubated in ACM from animals fed SFA (+159%) and 
      SFA/n-3 PUFA (+151%). Glucose transport was impaired (P </= 0.05) in PALM muscles 
      but preserved in ACM groups. Acute exposure of muscle to fatty acid modulated 
      adipokines affects skeletal muscle inflammatory gene expression but not insulin 
      sensitivity.
FAU - Tishinsky, Justine M
AU  - Tishinsky JM
AD  - Department of Human Health and Nutritional Sciences, University of Guelph, 
      Guelph, ON N1G 2W1, Canada.
FAU - De Boer, Anna A
AU  - De Boer AA
FAU - Dyck, David J
AU  - Dyck DJ
FAU - Robinson, Lindsay E
AU  - Robinson LE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130618
PL  - Canada
TA  - Appl Physiol Nutr Metab
JT  - Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition 
      et metabolisme
JID - 101264333
RN  - 0 (Adipokines)
RN  - 0 (Dietary Fats)
RN  - 0 (Fatty Acids)
SB  - IM
MH  - Adipokines/*metabolism
MH  - Animals
MH  - Dietary Fats/*pharmacology
MH  - Fatty Acids/*pharmacology
MH  - Female
MH  - Inflammation/*metabolism
MH  - *Insulin Resistance
MH  - Intra-Abdominal Fat/*metabolism
MH  - Muscle, Skeletal/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2014/01/05 06:00
MHDA- 2016/05/27 06:00
CRDT- 2014/01/04 06:00
PHST- 2014/01/04 06:00 [entrez]
PHST- 2014/01/05 06:00 [pubmed]
PHST- 2016/05/27 06:00 [medline]
AID - 10.1139/apnm-2013-0135 [doi]
PST - ppublish
SO  - Appl Physiol Nutr Metab. 2014 Jan;39(1):28-37. doi: 10.1139/apnm-2013-0135. Epub 
      2013 Jun 18.