PMID- 24384983
OWN - NLM
STAT- MEDLINE
DCOM- 20141107
LR  - 20211203
IS  - 1439-6327 (Electronic)
IS  - 1439-6319 (Linking)
VI  - 114
IP  - 4
DP  - 2014 Apr
TI  - Whey protein intake after resistance exercise activates mTOR signaling in a 
      dose-dependent manner in human skeletal muscle.
PG  - 735-42
LID - 10.1007/s00421-013-2812-7 [doi]
AB  - PURPOSE: Protein ingestion after resistance exercise increases muscle protein 
      synthesis (MPS) in a dose-dependent manner. However, the molecular mechanism(s) 
      for the dose-dependency of MPS remains unclear. This study aimed to determine the 
      dose response of mammalian target of rapamycin (mTOR) signaling in muscle with 
      ingestion of protein after resistance exercise. METHODS: Fifteen male subjects 
      performed four sets of six unilateral isokinetic concentric knee extensions. 
      Immediately after exercise, eight subjects consumed water only. The other seven 
      subjects, in a randomized-order crossover design, took either a 10 [3.6 g 
      essential amino acids (EAA)] or 20 g (7.1 g EAA) solution of whey protein. Muscle 
      biopsies from the vastus lateralis muscle were taken 30 min before and 1 h after 
      resistance exercise. Phosphorylation of Akt (Ser473), mTOR (Ser2448), 4E-BP1 
      (Thr37/46), and S6K1 (Thr389) was measured by western blotting. RESULTS: 
      Concentric knee extension exercise alone did not increase phosphorylation of Akt 
      and mTOR 1 h after exercise, but ingesting protein after exercise significantly 
      increased the phosphorylation of Akt and mTOR in a dose-dependent manner (P < 
      0.05). 4E-BP1 phosphorylation significantly decreased after resistance exercise 
      (P < 0.05), but subjects who took 10 or 20 g of protein after exercise showed 
      increased 4E-BP1 from post-exercise dephosphorylation (P < 0.05). S6K1 
      phosphorylation significantly increased after resistance exercise (P < 0.05), and 
      20 g of protein further increased S6K1 phosphorylation compared with ingestion of 
      10 g (P < 0.05). CONCLUSIONS: These findings suggest that whey protein intake 
      after resistance exercise activates mTOR signaling in a dose-dependent manner in 
      untrained men.
FAU - Kakigi, Ryo
AU  - Kakigi R
AD  - Institute of Health and Sports Science & Medicine, Juntendo University, 1-1 
      Hiragagakuendai, Inzai, Chiba, 270-1695, Japan.
FAU - Yoshihara, Toshinori
AU  - Yoshihara T
FAU - Ozaki, Hayao
AU  - Ozaki H
FAU - Ogura, Yuji
AU  - Ogura Y
FAU - Ichinoseki-Sekine, Noriko
AU  - Ichinoseki-Sekine N
FAU - Kobayashi, Hiroyuki
AU  - Kobayashi H
FAU - Naito, Hisashi
AU  - Naito H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140103
PL  - Germany
TA  - Eur J Appl Physiol
JT  - European journal of applied physiology
JID - 100954790
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Amino Acids, Essential)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (EIF4EBP1 protein, human)
RN  - 0 (Milk Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Whey Proteins)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (ribosomal protein S6 kinase, 70kD, polypeptide 1)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Administration, Oral
MH  - Amino Acids, Essential/metabolism
MH  - Cell Cycle Proteins
MH  - Cross-Over Studies
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Knee/physiology
MH  - Male
MH  - Milk Proteins/administration & dosage/*pharmacology
MH  - Muscle, Skeletal/*drug effects/metabolism/physiology
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - *Resistance Training
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Whey Proteins
MH  - Young Adult
EDAT- 2014/01/05 06:00
MHDA- 2014/11/08 06:00
CRDT- 2014/01/04 06:00
PHST- 2013/08/09 00:00 [received]
PHST- 2013/12/21 00:00 [accepted]
PHST- 2014/01/04 06:00 [entrez]
PHST- 2014/01/05 06:00 [pubmed]
PHST- 2014/11/08 06:00 [medline]
AID - 10.1007/s00421-013-2812-7 [doi]
PST - ppublish
SO  - Eur J Appl Physiol. 2014 Apr;114(4):735-42. doi: 10.1007/s00421-013-2812-7. Epub 
      2014 Jan 3.