PMID- 24385363
OWN - NLM
STAT- MEDLINE
DCOM- 20140917
LR  - 20211021
IS  - 1976-3794 (Electronic)
IS  - 1225-8873 (Linking)
VI  - 51
IP  - 6
DP  - 2013 Dec
TI  - Inhibitory effect of chlorophyllin on the Propionibacterium acnes-induced 
      chemokine expression.
PG  - 844-9
LID - 10.1007/s12275-013-3015-y [doi]
AB  - Chlorophyllin (CHL), a chlorophyll-derivative, exhibits several beneficial 
      properties, including antibacterial, antioxidant, and anticancer activities. 
      However, its antibacterial and anti-inflammatory activities against 
      Propionibacterium acnes have not been described. The antibacterial activity of 
      this compound was evaluated in vitro using the broth microdilution method. CHL 
      had an inhibitory effect on the growth of P. acnes (MIC = 100 muM). In a real-time 
      reverse transcription-polymerase chain reaction and an enzyme-linked 
      immunosorbent assay, CHL significantly decreased interleukin-8 (IL-8) and 
      monocyte chemoattractant protein-1 (MCP-1) production in a dose-dependent manner, 
      decreasing both mRNA and protein levels for these chemokines in THP-1 cells 
      indicating the anti-inflammatory effects of it. To investigate the molecular 
      mechanisms underlying the anti-inflammatory properties of CHL in THP-1 cells 
      stimulated by P. acnes, we used western blotting to analyze the effect of CHL on 
      activation of the nuclear factor (NF)-kappaB. CHL inhibited P. acnes-induced IL-8 and 
      MCP-1 production via blockade of NF-kappaB activation in THP-1 cells. Therefore, 
      based on these results, we suggest that CHL is a useful agent to control the 
      growth of P. acnes involved in acne inflammation and prevent acne.
FAU - Kang, Mi-Sun
AU  - Kang MS
AD  - Department of Microbiology, School of Medicine, Chonnam National University, 
      Gwangju, 501-746, Republic of Korea.
FAU - Kim, Jin-Hee
AU  - Kim JH
FAU - Shin, Boo-Ahn
AU  - Shin BA
FAU - Lee, Hyun-Chul
AU  - Lee HC
FAU - Kim, Youn-Shin
AU  - Kim YS
FAU - Lim, Hae-Soon
AU  - Lim HS
FAU - Oh, Jong-Suk
AU  - Oh JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131219
PL  - Korea (South)
TA  - J Microbiol
JT  - Journal of microbiology (Seoul, Korea)
JID - 9703165
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Chlorophyllides)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - EEM82VOY7C (chlorophyllin)
SB  - IM
EIN - J Microbiol. 2014 Feb;52(2):184
MH  - Acne Vulgaris/genetics/immunology/microbiology
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Cell Line
MH  - Chemokine CCL2/genetics/immunology
MH  - Chemokines/*genetics/immunology
MH  - Chlorophyllides/*pharmacology
MH  - Down-Regulation/drug effects
MH  - Gram-Positive Bacterial Infections/*genetics/immunology/microbiology
MH  - Humans
MH  - Interleukin-8/genetics/immunology
MH  - NF-kappa B/genetics/immunology
MH  - Propionibacterium acnes/*drug effects/physiology
EDAT- 2014/01/05 06:00
MHDA- 2014/09/18 06:00
CRDT- 2014/01/04 06:00
PHST- 2013/01/08 00:00 [received]
PHST- 2013/06/10 00:00 [accepted]
PHST- 2014/01/04 06:00 [entrez]
PHST- 2014/01/05 06:00 [pubmed]
PHST- 2014/09/18 06:00 [medline]
AID - 10.1007/s12275-013-3015-y [doi]
PST - ppublish
SO  - J Microbiol. 2013 Dec;51(6):844-9. doi: 10.1007/s12275-013-3015-y. Epub 2013 Dec 
      19.