PMID- 24385485
OWN - NLM
STAT- MEDLINE
DCOM- 20140227
LR  - 20211021
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 203
IP  - 4
DP  - 2013 Nov 25
TI  - The association of microtubules with tight junctions is promoted by cingulin 
      phosphorylation by AMPK.
PG  - 605-14
LID - 10.1083/jcb.201304194 [doi]
AB  - Epithelial cells characteristically have noncentrosomal microtubules that are 
      arranged in the apicobasal direction. In this paper, we examined cell sheets 
      formed by an epithelial (Eph4) cell line by structure illumination microscopy and 
      found a previously not clearly described planar apical network of noncentrosomal 
      microtubules (MTs) in which the sides of the MT bundles were associated with 
      tight junctions (TJs). In a gel overlay assay with taxol-stabilized MTs, cingulin 
      showed strong binding to MTs, and a domain analysis showed that this binding 
      occurred through cingulin's N-terminal region. The association of planar apical 
      MTs with TJs was compromised by cingulin knockdown (KD) or the expression of 
      dephosphomimetic mutants of cingulin at its adenosine monophosphate-activated 
      protein kinase (AMPK) target sites, whereas phosphorylation at these sites 
      facilitated cingulin-tubulin binding. In addition, although wild-type colonies 
      formed spheres in 3D culture, the cingulin KD cells had anisotropic shapes. These 
      findings collectively suggest that the regulated cingulin-MT association has a 
      specific role in TJ-related epithelial morphogenesis that is sensitive to 
      metabolic homeostasis-related AMPK activity.
FAU - Yano, Tomoki
AU  - Yano T
AD  - Laboratory of Biological Science, Graduate School of Frontier Biosciences and 
      Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
FAU - Matsui, Takeshi
AU  - Matsui T
FAU - Tamura, Atsushi
AU  - Tamura A
FAU - Uji, Masami
AU  - Uji M
FAU - Tsukita, Sachiko
AU  - Tsukita S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Cgn protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Tubulin)
RN  - 17885-08-4 (Phosphoserine)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Calcium/metabolism
MH  - Cell Polarity
MH  - Centrosome/metabolism
MH  - Epithelial Cells/cytology/metabolism
MH  - Epithelium/growth & development/metabolism
MH  - Fluorescence Resonance Energy Transfer
MH  - Fluorescent Antibody Technique
MH  - Gene Knockdown Techniques
MH  - HEK293 Cells
MH  - Humans
MH  - Intercellular Junctions/metabolism
MH  - Membrane Proteins/chemistry/*metabolism
MH  - Mice
MH  - Microtubules/*metabolism
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - Morphogenesis
MH  - Phosphorylation
MH  - Phosphoserine/metabolism
MH  - Protein Structure, Tertiary
MH  - Sus scrofa
MH  - Tight Junctions/*metabolism
MH  - Tubulin/metabolism
PMC - PMC3840929
EDAT- 2014/01/05 06:00
MHDA- 2014/02/28 06:00
PMCR- 2014/05/25
CRDT- 2014/01/04 06:00
PHST- 2014/01/04 06:00 [entrez]
PHST- 2014/01/05 06:00 [pubmed]
PHST- 2014/02/28 06:00 [medline]
PHST- 2014/05/25 00:00 [pmc-release]
AID - jcb.201304194 [pii]
AID - 201304194 [pii]
AID - 10.1083/jcb.201304194 [doi]
PST - ppublish
SO  - J Cell Biol. 2013 Nov 25;203(4):605-14. doi: 10.1083/jcb.201304194.