PMID- 24386425
OWN - NLM
STAT- MEDLINE
DCOM- 20150223
LR  - 20220311
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 12
DP  - 2013
TI  - Microarray gene expression analysis of tumorigenesis and regional lymph node 
      metastasis in laryngeal squamous cell carcinoma.
PG  - e84854
LID - 10.1371/journal.pone.0084854 [doi]
LID - e84854
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is the most common type in 
      head and neck squamous cell carcinoma (HNSCC), and the development and 
      progression of LSCC are multistep processes accompanied by changes of molecular 
      biology. OBJECTIVE: The purpose of this study was to investigate the molecular 
      basis of tumorigenesis and regional lymph node metastasis in LSCC, and provide a 
      set of genes that may be useful for the development of novel diagnostic markers 
      and/or more effective therapeutic strategies. METHODS: A total number of 10 
      patients who underwent surgery for primary laryngeal squamous cell carcinoma were 
      recruited for microarray analysis. LSCC tissues compared with corresponding 
      adjacent non-neoplastic tissues were analysed by Illumina mRNA microarrays, and 
      LSCC tissues with regional lymph node metastasis and LSCC tissues without 
      regional lymph node metastasis were analyzed in the same manner. The most 
      frequently differently expressed genes screened by microarrays were also 
      validated by qRT-PCR in another 42 patients diagnosed for LSCC. RESULTS: Analysed 
      by Illumina mRNA microarrays, there were 361 genes significantly related to 
      tumorigenesis while 246 genes significantly related to regional lymph node 
      metastasis in LSCC. We found that the six genes (CDK1, CDK2, CDK4, MCM2, MCM3, 
      MCM4) were most frequently differently expressed functional genes related to 
      tumorigenesis while eIF3a and RPN2 were most frequently differently expressed 
      functional genes related to regional lymph node metastasis in LSCC. The 
      expressions of these genes were also validated by qRT-PCR. CONCLUSIONS: The 
      research revealed a gene expression signature of tumorigenesis and regional lymph 
      node metastasis in laryngeal squamous cell carcinoma. Of the total, the 
      deregulation of several genes (CDK1, CDK2, CDK4, MCM2, MCM3, MCM4, EIF3a and 
      RPN2) were potentially associated with disease development and progression. The 
      result will contribute to the understanding of the molecular basis of LSCC and 
      help to improve diagnosis and treatment.
FAU - Lian, Meng
AU  - Lian M
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
FAU - Fang, Jugao
AU  - Fang J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
FAU - Han, Demin
AU  - Han D
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China ; Key Laboratory of 
      Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing 
      Institute of Otorhinolaryngology, Beijing, China.
FAU - Ma, Hongzhi
AU  - Ma H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
FAU - Feng, Ling
AU  - Feng L
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
FAU - Wang, Ru
AU  - Wang R
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
FAU - Yang, Fan
AU  - Yang F
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, China.
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131226
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Aged
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Transformation, Neoplastic/*metabolism/pathology
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Lymphatic Metastasis
MH  - Middle Aged
MH  - Neoplasm Proteins/*biosynthesis
MH  - Oligonucleotide Array Sequence Analysis
PMC - PMC3873425
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/01/05 06:00
MHDA- 2015/02/24 06:00
PMCR- 2013/12/26
CRDT- 2014/01/04 06:00
PHST- 2013/08/29 00:00 [received]
PHST- 2013/11/19 00:00 [accepted]
PHST- 2014/01/04 06:00 [entrez]
PHST- 2014/01/05 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PHST- 2013/12/26 00:00 [pmc-release]
AID - PONE-D-13-35543 [pii]
AID - 10.1371/journal.pone.0084854 [doi]
PST - epublish
SO  - PLoS One. 2013 Dec 26;8(12):e84854. doi: 10.1371/journal.pone.0084854. 
      eCollection 2013.