PMID- 24386942
OWN - NLM
STAT- MEDLINE
DCOM- 20150408
LR  - 20140930
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 62
IP  - 3
DP  - 2014 Jan 22
TI  - Glycyrrhetinic acid suppressed NF-kappaB activation in TNF-alpha-induced hepatocytes.
PG  - 618-25
LID - 10.1021/jf405352g [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a crucial inflammatory cytokine when 
      hepatocytes are damaged. Glycyrrhiza uralensis Fisch. (Chinese licorice) has been 
      widely used in Chinese herbal prescriptions for the treatment of liver diseases 
      and as a food additive. Nuclear factor-kappa B (NF-kappaB) reporter gene assay in 
      TNF-alpha-induced HepG2 was used as a screening platform. IkappaBalpha phosphorylation and 
      p65 translocation were measured by Western blotting, and nitric oxide (NO) 
      production and inducible nitric oxide synthase (iNOS) gene expression were 
      further confirmed in rat primary hepatocytes. Results showed that TNF-alpha enhanced 
      NF-kappaB activity was significantly attenuated by glycyrrhetinic acid in a 
      concentration-dependent manner in the NF-kappaB reporter gene assay. Glycyrrhetinic 
      acid decreased the gene expression of iNOS through inhibited IkappaBalpha phosphorylation 
      and p65 translocation in protein level. Furthermore, NO production and iNOS 
      expression were reduced by glycyrrhetinic acid in TNF-alpha-induced rat primary 
      hepatocytes. These results suggest that glycyrrhetinic acid may provide 
      hepatoprotection against chronic liver inflammation through attenuating NF-kappaB 
      activation to alleviate the inflammation.
FAU - Chen, Hong-Jhang
AU  - Chen HJ
AD  - National Research Institute of Chinese Medicine, Ministry of Health and Welfare , 
      Taipei, Taiwan.
FAU - Kang, Shih-Pei
AU  - Kang SP
FAU - Lee, I-Jung
AU  - Lee IJ
FAU - Lin, Yun-Lian
AU  - Lin YL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140110
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - P540XA09DR (Glycyrrhetinic Acid)
SB  - IM
MH  - Animals
MH  - Down-Regulation/drug effects
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Glycyrrhetinic Acid/*pharmacology
MH  - Glycyrrhiza uralensis/*chemistry
MH  - Hepatocytes/*drug effects/metabolism
MH  - Humans
MH  - I-kappa B Proteins/metabolism
MH  - NF-kappa B/*metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Phosphorylation/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - Tumor Necrosis Factor-alpha/*metabolism
OTO - NOTNLM
OT  - Glycyrrhiza uralensis
OT  - NF-kappaB
OT  - TNF-alpha
OT  - glycyrrhetinic acid
OT  - hepatocytes
EDAT- 2014/01/07 06:00
MHDA- 2015/04/09 06:00
CRDT- 2014/01/07 06:00
PHST- 2014/01/07 06:00 [entrez]
PHST- 2014/01/07 06:00 [pubmed]
PHST- 2015/04/09 06:00 [medline]
AID - 10.1021/jf405352g [doi]
PST - ppublish
SO  - J Agric Food Chem. 2014 Jan 22;62(3):618-25. doi: 10.1021/jf405352g. Epub 2014 
      Jan 10.