PMID- 24388047
OWN - NLM
STAT- MEDLINE
DCOM- 20150207
LR  - 20220408
IS  - 1873-569X (Electronic)
IS  - 0923-1811 (Linking)
VI  - 74
IP  - 1
DP  - 2014 Apr
TI  - Simple and rapid detection of HLA-A*31:01 for prediction of carbamazepine-induced 
      hypersensitivity using loop-mediated isothermal amplification method.
PG  - 88-92
LID - S0923-1811(13)00383-6 [pii]
LID - 10.1016/j.jdermsci.2013.11.013 [doi]
AB  - BACKGROUND: Carbamazepine (CBZ), which is widely used in management of epilepsy 
      or neuropathic pain, causes fatal severe cutaneous adverse reactions (SCARs). 
      CBZ-induced SCARs are known to occur in strong association with human leukocyte 
      antigen (HLA)-A*31:01 in Japanese and European populations. HLA genotyping is 
      currently used to detect human HLA-A*31:01. OBJECTIVE: To establish a simple and 
      rapid screening assay specific for HLA-A*31:01, the loop-mediated isothermal 
      amplification (LAMP) method was employed on a sample Japanese population. 
      METHODS: A set of LAMP primers targeting exon 2 of HLA-A*31:01 were designed. 
      Thirty-two clinical samples including the representative HLA-A allele in Japan 
      were used to assess the specificity of LAMP primers in the detection of 
      HLA-A*31:01. RESULTS: The HLA-A*31:01-specific LAMP assay showed consistency with 
      polymerase chain reaction reverse sequence-specific oligonucleotide probe 
      (PCR-rSSO) and polymerase chain reaction-sequence based typing (PCR-SBT) results. 
      CONCLUSION: High sensitivity and specificity of the HLA-A*31:01 LAMP assay was 
      confirmed. Considering its convenience, the assay can be widely used to screen 
      patients at high genetic risk of CBZ-induced SCARs.
CI  - Copyright (c) 2014 Japanese Society for Investigative Dermatology. Published by 
      Elsevier Ireland Ltd. All rights reserved.
FAU - Niihara, Hiroyuki
AU  - Niihara H
AD  - Department of Dermatology, Shimane University Hospital, Izumo, Japan. Electronic 
      address: ofcourse@med.shimane-u.ac.jp.
FAU - Kohno, Kunie
AU  - Kohno K
AD  - Center for Community-Based Health Research and Education (COHRE), Organization 
      for the Promotion of Project Research, Shimane University, Izumo, Japan.
FAU - Taketani, Takeshi
AU  - Taketani T
AD  - Department of Pediatrics, Shimane University Hospital, Izumo, Japan.
FAU - Kaneko, Sakae
AU  - Kaneko S
AD  - Department of Dermatology, Shimane University Hospital, Izumo, Japan.
FAU - Ito, Takafumi
AU  - Ito T
AD  - Section of Nephrology, Department of Internal Medicine, Shimane University 
      Hospital, Izumo, Japan.
FAU - Sugamori, Takashi
AU  - Sugamori T
AD  - Section of Cardiovascular Internal Medicine, Department of Internal Medicine, 
      Shimane University Hospital, Izumo, Japan.
FAU - Takahashi, Nobuyuki
AU  - Takahashi N
AD  - Section of Cardiovascular Internal Medicine, Department of Internal Medicine, 
      Shimane University Hospital, Izumo, Japan.
FAU - Miyaoka, Tuyoshi
AU  - Miyaoka T
AD  - Department of Psychiatry, Shimane University Hospital, Izumo, Japan.
FAU - Okazaki, Shihoh
AU  - Okazaki S
AD  - Department of Psychiatry, Shimane University Hospital, Izumo, Japan.
FAU - Yasuda, Hideaki
AU  - Yasuda H
AD  - Department of Psychiatry, Shimane University Hospital, Izumo, Japan.
FAU - Furuya, Motohide
AU  - Furuya M
AD  - Department of Psychiatry, Shimane University Hospital, Izumo, Japan.
FAU - Nagahama, Michiharu
AU  - Nagahama M
AD  - Department of Psychiatry, Shimane University Hospital, Izumo, Japan.
FAU - Morita, Eishin
AU  - Morita E
AD  - Department of Dermatology, Shimane University Hospital, Izumo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131216
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (Anticonvulsants)
RN  - 0 (DNA Primers)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*31:01 antigen)
RN  - 33CM23913M (Carbamazepine)
SB  - IM
MH  - Alleles
MH  - Anticonvulsants/adverse effects
MH  - Base Sequence
MH  - Carbamazepine/*adverse effects
MH  - DNA Primers/genetics
MH  - Drug Hypersensitivity/*genetics/mortality
MH  - Gene Frequency
MH  - Genotype
MH  - HLA-A Antigens/*analysis
MH  - Humans
MH  - Japan
MH  - Molecular Sequence Data
MH  - *Nucleic Acid Amplification Techniques
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Skin/drug effects
OTO - NOTNLM
OT  - Carbamazepine
OT  - Fatal severe cutaneous adverse reactions
OT  - Human leukocyte antigen-A*31:01
OT  - Loop-mediated isothermal amplification
EDAT- 2014/01/07 06:00
MHDA- 2015/02/11 06:00
CRDT- 2014/01/07 06:00
PHST- 2013/05/18 00:00 [received]
PHST- 2013/10/31 00:00 [revised]
PHST- 2013/11/27 00:00 [accepted]
PHST- 2014/01/07 06:00 [entrez]
PHST- 2014/01/07 06:00 [pubmed]
PHST- 2015/02/11 06:00 [medline]
AID - S0923-1811(13)00383-6 [pii]
AID - 10.1016/j.jdermsci.2013.11.013 [doi]
PST - ppublish
SO  - J Dermatol Sci. 2014 Apr;74(1):88-92. doi: 10.1016/j.jdermsci.2013.11.013. Epub 
      2013 Dec 16.