PMID- 24389036
OWN - NLM
STAT- MEDLINE
DCOM- 20141028
LR  - 20151119
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 98
DP  - 2014 Mar
TI  - Extracellular protein biomarkers for the characterization of enterohemorrhagic 
      and enteroaggregative Escherichia coli strains.
PG  - 76-83
LID - S0167-7012(13)00395-3 [pii]
LID - 10.1016/j.mimet.2013.12.017 [doi]
AB  - The extracellular proteins (ECPs) of enterohemorrhagic Escherichia coli (EHEC) 
      can cause hemorrhagic colitis which may cause life threatening hemolytic-uremic 
      syndrome, while that of enteroaggregative E. coli (EAEC) can clump to intestinal 
      membranes. Liquid chromatography-electrospray ionization-tandem mass spectrometry 
      based proteomics is used to evaluate a preliminary study on the extracellular and 
      whole cell protein extracts associated with E. coli strain pathogenicity. 
      Proteomics analysis, which is independent of genomic sequencing, of EAEC O104:H4 
      (unsequenced genome) identified a number of proteins. Proteomics of EHEC O104:H4, 
      causative agent of the Germany outbreak, showed a closest match with E. coli 
      E55989, in agreement with genomic studies. Dendrogram analysis separated EHEC 
      O157:H7 and EHEC/EAEC O104:H4. ECP analysis compared to that of whole cell 
      processing entails few steps and convenient experimental extraction procedures. 
      Bacterial characterization results are promising in exploring the impact of 
      environmental conditions on E. coli ECP biomarkers with a few relatively 
      straightforward protein extraction steps.
CI  - Published by Elsevier B.V.
FAU - Jabbour, Rabih E
AU  - Jabbour RE
AD  - Research and Technology Directorate, Edgewood Chemical Biological Center, US Army 
      Aberdeen Proving Ground, MD 21010, United States.
FAU - Deshpande, Samir V
AU  - Deshpande SV
AD  - Science and Technology Corporation Edgewood, MD 21040, United States.
FAU - McCubbin, Patrick E
AU  - McCubbin PE
AD  - Optimetrics, Inc., Abingdon, MD 21009, United States.
FAU - Wright, James D
AU  - Wright JD
AD  - Research and Technology Directorate, Edgewood Chemical Biological Center, US Army 
      Aberdeen Proving Ground, MD 21010, United States.
FAU - Wade, Mary Margaret
AU  - Wade MM
AD  - Research and Technology Directorate, Edgewood Chemical Biological Center, US Army 
      Aberdeen Proving Ground, MD 21010, United States.
FAU - Snyder, A Peter
AU  - Snyder AP
AD  - Research and Technology Directorate, Edgewood Chemical Biological Center, US Army 
      Aberdeen Proving Ground, MD 21010, United States. Electronic address: 
      arnold.p.snyder.civ@mail.mil.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20131231
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Biomarkers)
RN  - 0 (Escherichia coli Proteins)
SB  - IM
MH  - Biomarkers/*chemistry/metabolism
MH  - Chromatography, Liquid/methods
MH  - Disease Outbreaks
MH  - Escherichia coli/genetics/*metabolism
MH  - Escherichia coli Infections/*diagnosis/genetics/metabolism/*microbiology
MH  - Escherichia coli O157/genetics/*metabolism
MH  - Escherichia coli Proteins/genetics/*metabolism
MH  - Genomics/methods
MH  - Germany
MH  - Proteomics/methods
MH  - Spectrometry, Mass, Electrospray Ionization/methods
OTO - NOTNLM
OT  - Electrospray
OT  - Enteroaggregative E. coli
OT  - Enterohemorrhagic E. coli
OT  - Extracellular proteins
OT  - Liquid chromatography-tandem mass spectrometry
OT  - Proteomics
EDAT- 2014/01/07 06:00
MHDA- 2014/10/29 06:00
CRDT- 2014/01/07 06:00
PHST- 2013/09/10 00:00 [received]
PHST- 2013/12/18 00:00 [revised]
PHST- 2013/12/20 00:00 [accepted]
PHST- 2014/01/07 06:00 [entrez]
PHST- 2014/01/07 06:00 [pubmed]
PHST- 2014/10/29 06:00 [medline]
AID - S0167-7012(13)00395-3 [pii]
AID - 10.1016/j.mimet.2013.12.017 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2014 Mar;98:76-83. doi: 10.1016/j.mimet.2013.12.017. Epub 
      2013 Dec 31.