PMID- 24391310
OWN - NLM
STAT- MEDLINE
DCOM- 20141014
LR  - 20211021
IS  - 1526-6702 (Electronic)
IS  - 0730-2347 (Print)
IS  - 0730-2347 (Linking)
VI  - 40
IP  - 5
DP  - 2013
TI  - Induction of early biomarkers in a thrombus-induced sheep model of ischemic heart 
      failure.
PG  - 511-20
AB  - The levels of brain natriuretic peptide (BNP) and monocyte chemoattractant 
      protein-1 (MCP-1) are known to be increased in the sera of subjects with heart 
      failure. Existing models do not account for the biomass of thrombus that occurs 
      in patients undergoing myocardial infarction. In this study, we compared the 
      expressions of sheep-derived genes for BNP, MCP-1, and atrial natriuretic peptide 
      in a new large-animal model of thrombus-induced heart failure. Thrombus of 
      autologous platelets was injected directly into the left circumflex coronary 
      arteries of sheep. Cardiac ischemic injury was evaluated by troponin I levels, 
      and heart failure progression was monitored with the aid of echocardiogram-based 
      evaluation. After outlined time intervals, the sheep were killed and their hearts 
      excised for tissue sampling. Reverse transcription polymerase chain reaction, 
      Western blot, and enzyme-linked immunosorbent assay (ELISA) tests were performed 
      to establish gene and protein expressions. At 72 hours after embolization, 
      myocardial BNP and MCP-1 expressions had increased significantly in the ischemic 
      region, compared either with the nonischemic region or with tissue from healthy 
      sheep. As heart failure progressed to 90 days after embolization, the expression 
      of BNP in the ischemic region decreased, whereas its expression in the 
      nonischemic region increased several fold. In contrast, MCP-1 gene expression 
      showed no changes in either tissue after 90 days of embolization. Plasma levels 
      of BNP, determined by Western blot and ELISA, also correlated with the 
      gene-expression studies. Our results show regional changes in BNP and MCP-1, as 
      well as differences in the expression of these 2 genes.
FAU - Chandrakala, Aluganti N
AU  - Chandrakala AN
AD  - Division of Cardiac Surgery, Department of Surgery, The Ohio State University 
      Medical Center, Columbus, Ohio 43210.
FAU - Kwiatkowski, Pawel
AU  - Kwiatkowski P
AD  - Division of Cardiac Surgery, Department of Surgery, The Ohio State University 
      Medical Center, Columbus, Ohio 43210.
FAU - Sai-Sudhakar, Chittoor B
AU  - Sai-Sudhakar CB
AD  - Division of Cardiac Surgery, Department of Surgery, The Ohio State University 
      Medical Center, Columbus, Ohio 43210.
FAU - Sun, Benjamin
AU  - Sun B
AD  - Division of Cardiac Surgery, Department of Surgery, The Ohio State University 
      Medical Center, Columbus, Ohio 43210.
FAU - Phillips, Angela
AU  - Phillips A
AD  - Division of Cardiac Surgery, Department of Surgery, The Ohio State University 
      Medical Center, Columbus, Ohio 43210.
FAU - Parthasarathy, Sampath
AU  - Parthasarathy S
AD  - Division of Cardiac Surgery, Department of Surgery, The Ohio State University 
      Medical Center, Columbus, Ohio 43210.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tex Heart Inst J
JT  - Texas Heart Institute journal
JID - 8214622
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 114471-18-0 (Natriuretic Peptide, Brain)
SB  - IM
MH  - Animals
MH  - Biomarkers/*blood
MH  - Blotting, Western
MH  - Chemokine CCL2/blood/genetics
MH  - Coronary Thrombosis/*blood/complications/genetics
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Follow-Up Studies
MH  - Gene Expression Regulation
MH  - Heart Failure/*blood/etiology/genetics
MH  - Natriuretic Peptide, Brain/blood/genetics
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sheep, Domestic
MH  - Time Factors
PMC - PMC3853808
OTO - NOTNLM
OT  - Atrial natriuretic peptide
OT  - C-reactive protein
OT  - biological markers/blood
OT  - blood platelets
OT  - brain
OT  - chemokines/blood
OT  - disease models/animal
OT  - heart failure
OT  - monocyte chemoattractant protein-1/blood
OT  - myocardial ischemia
OT  - natriuretic peptide
OT  - sheep
OT  - stroke volume
OT  - ventricular dysfunction, left/etiology
EDAT- 2014/01/07 06:00
MHDA- 2014/10/15 06:00
PMCR- 2013/01/01
CRDT- 2014/01/07 06:00
PHST- 2014/01/07 06:00 [entrez]
PHST- 2014/01/07 06:00 [pubmed]
PHST- 2014/10/15 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 0010801-201312000-00004 [pii]
PST - ppublish
SO  - Tex Heart Inst J. 2013;40(5):511-20.