PMID- 24391549 OWN - NLM STAT- MEDLINE DCOM- 20140408 LR - 20220129 IS - 1662-5110 (Print) IS - 1662-5110 (Electronic) IS - 1662-5110 (Linking) VI - 7 DP - 2013 TI - Regulation of thalamocortical axon branching by BDNF and synaptic vesicle cycling. PG - 202 LID - 10.3389/fncir.2013.00202 [doi] LID - 202 AB - During development, axons form branches in response to extracellular molecules. Little is known about the underlying molecular mechanisms. Here, we investigate how neurotrophin-induced axon branching is related to synaptic vesicle cycling for thalamocortical axons. The exogenous application of brain-derived neurotrophic factor (BDNF) markedly increased axon branching in thalamocortical co-cultures, while removal of endogenous BDNF reduced branching. Over-expression of a C-terminal fragment of AP180 that inhibits clathrin-mediated endocytosis affected the laminar distribution and the number of branch points. A dominant-negative synaptotagmin mutant that selectively targets synaptic vesicle cycling, strongly suppressed axon branching. Moreover, axons expressing the mutant synaptotagmin were resistant to the branch-promoting effect of BDNF. These results suggest that synaptic vesicle cycling might regulate BDNF induced branching during the development of the axonal arbor. FAU - Granseth, Bjorn AU - Granseth B AD - Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University Suita, Osaka, Japan ; Division of Cell Biology, Department of Clinical and Experimental Medicine, Linkoping University Linkoping, Sweden. FAU - Fukushima, Yuichi AU - Fukushima Y AD - Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University Suita, Osaka, Japan. FAU - Sugo, Noriuki AU - Sugo N AD - Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University Suita, Osaka, Japan. FAU - Lagnado, Leon AU - Lagnado L AD - Sussex Neuroscience, School of Life Sciences, University of Sussex Brighton, UK. FAU - Yamamoto, Nobuhiko AU - Yamamoto N AD - Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University Suita, Osaka, Japan. LA - eng GR - MC_U105178794/MRC_/Medical Research Council/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20131220 PL - Switzerland TA - Front Neural Circuits JT - Frontiers in neural circuits JID - 101477940 RN - 0 (Brain-Derived Neurotrophic Factor) SB - IM MH - Animals MH - Axons/drug effects/*metabolism MH - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology MH - Cells, Cultured MH - Cerebral Cortex/drug effects/*metabolism MH - Endocytosis/drug effects/physiology MH - Neurons/drug effects/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Synaptic Vesicles/drug effects/*metabolism MH - Thalamus/drug effects/*metabolism PMC - PMC3868945 OTO - NOTNLM OT - axon OT - branching OT - development OT - endocytosis OT - neocortex OT - neurotrophin OT - synapse OT - thalamus EDAT- 2014/01/07 06:00 MHDA- 2014/04/09 06:00 PMCR- 2013/01/01 CRDT- 2014/01/07 06:00 PHST- 2013/07/31 00:00 [received] PHST- 2013/12/04 00:00 [accepted] PHST- 2014/01/07 06:00 [entrez] PHST- 2014/01/07 06:00 [pubmed] PHST- 2014/04/09 06:00 [medline] PHST- 2013/01/01 00:00 [pmc-release] AID - 10.3389/fncir.2013.00202 [doi] PST - epublish SO - Front Neural Circuits. 2013 Dec 20;7:202. doi: 10.3389/fncir.2013.00202. eCollection 2013.