PMID- 24392197
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 0277-786X (Print)
IS  - 0277-786X (Electronic)
IS  - 0277-786X (Linking)
VI  - 7568
DP  - 2010 Feb 26
TI  - Immersion Mirau interferometry for label-free live cell imaging in an 
      epi-illumination geometry.
LID - 10.1117/12.855651 [doi]
AB  - In cell biology studies it is often important to avoid the damaging effects 
      caused by fluorescent stains or UV-light. Immersion Mirau Interferometry (IMI) is 
      an epi-illumination label-free imaging technique developed at the Columbia 
      University Radiological Research Accelerator Facility. It is based on the 
      principles of phase-shifting interferometry (PSI) and represents a novel approach 
      for interferometric imaging of living cells in medium. To accommodate the use of 
      medium, a custom immersion Mirau interferometric attachment was designed and 
      built in-house. The space between the reference mirror and the beam splitter is 
      filled with liquid to ensure identical optical paths in the test and reference 
      arms. The interferometer is mountable onto a microscope objective. The greatest 
      limitation of standard PSI is the sensitivity to environmental vibrations, 
      because it requires consecutive acquisition of several interferograms. We are 
      developing Simultaneous Immersion Mirau Interferometry (SIMI), which facilitates 
      simultaneous acquisition of all interferograms and eliminates the effects of 
      vibration. Polarization optics, incorporated into the design, introduces a phase 
      delay to one of the components of the test beam. This enables simultaneous 
      creation and spatial separation of two interferograms, which are combined with 
      the background image to reconstruct the intensity map of the specimen. Our 
      results of imaging live and fixed cells with IMI and SIMI show that this system 
      produces images of a quality that is sufficient to perform targeted cellular 
      irradiation experiments.
FAU - Lyulko, Oleksandra V
AU  - Lyulko OV
AD  - Center for Radiological Research, Columbia University, New York, NY 10533.
FAU - Randers-Pehrson, Gerhard
AU  - Randers-Pehrson G
AD  - Center for Radiological Research, Columbia University, New York, NY 10533.
FAU - Brenner, David J
AU  - Brenner DJ
AD  - Center for Radiological Research, Columbia University, New York, NY 10533.
LA  - eng
GR  - P41 EB002033/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Proc SPIE Int Soc Opt Eng
JT  - Proceedings of SPIE--the International Society for Optical Engineering
JID - 101524122
PMC - PMC3877336
MID - NIHMS483923
OTO - NOTNLM
OT  - Interferometry
OT  - dynamic imaging
OT  - label-free imaging
OT  - live cell imaging.
OT  - no-stain imaging
OT  - phase shifting
OT  - polarization
OT  - simultaneous interferometric imaging
OT  - vibration
EDAT- 2010/02/26 00:00
MHDA- 2010/02/26 00:01
PMCR- 2013/12/31
CRDT- 2014/01/07 06:00
PHST- 2014/01/07 06:00 [entrez]
PHST- 2010/02/26 00:00 [pubmed]
PHST- 2010/02/26 00:01 [medline]
PHST- 2013/12/31 00:00 [pmc-release]
AID - 10.1117/12.855651 [doi]
PST - ppublish
SO  - Proc SPIE Int Soc Opt Eng. 2010 Feb 26;7568:10.1117/12.855651. doi: 
      10.1117/12.855651.