PMID- 24392624
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 31
IP  - 9
DP  - 2013 Sep
TI  - [Simultaneous determination of principal components and related substances of raw 
      material drug of ammonium glycyrrhizinate by reversed-phase high performance 
      liquid chromatography].
PG  - 869-74
AB  - An analytical method for the simultaneous determination of 18alpha-glycyrrhizic 
      acid, 18beta-glycyrrhizinic acid, related substances A and B and drug quality 
      standard by reversed-phase high performance liquid chromatography (RP-HPLC) was 
      established. The assay was carried out on a Durashell-C18 column (250 mm x 4.6 
      mm, 5 microm) with 10 mmol/L ammonium perchlorate (the pH value was adjusted to 
      8.20 with ammonia)-methanol (48:52, v/v) as mobile phase at a flow rate of 0.80 
      mL/min, and the detection wavelength was set at 254 nm. The column temperature 
      was 50 degrees C and the injection volume was 10 microL. Under the separation 
      conditions, the calibration curves of the analytes showed good linearities within 
      the mass concentrations of 0.50 -100 mg/L (r > 0.999 9). The detection limits for 
      18alpha-glycyrrhizic acid, 18beta-glycyrrhizinic acid, related substances A and B 
      were 0.15, 0.10, 0.10, 0.15 mg/L, respectively. The average recoveries were 
      between 97.32% and 99.33% (n = 3) with the relative standard deviations (RSDs) 
      between 0.05% and 1.06%. The method is sensitive, reproducible, and the results 
      are accurate and reliable. The method can be used for the determination of 
      principal components and related substances of ammonium glycyrrhizinate for the 
      quality control of raw material drug of ammonium glycyrrhizinate.
FAU - Zhao, Yanyan
AU  - Zhao Y
AD  - Medical Experimental Center, Hebei University, Baoding 071000, China. 
      zhaoyany606@tom.com
FAU - Liu, Liyan
AU  - Liu L
AD  - Medical Experimental Center, Hebei University, Baoding 071000, China.
FAU - Han, Yuanyuan
AU  - Han Y
AD  - Medical Experimental Center, Hebei University, Baoding 071000, China.
FAU - Li, Yueqiu
AU  - Li Y
AD  - Medical Experimental Center, Hebei University, Baoding 071000, China.
FAU - Wang, Yan
AU  - Wang Y
AD  - College of Chemistry and Environmental Science, Hebei University, Key Laboratory 
      of Analytical Science and Technology, Hebei Province, Key Laboratory of Chemical 
      Biology, Hebei Province, Baoding 071002, China.
FAU - Shi, Minjian
AU  - Shi M
AD  - College of Chemistry and Environmental Science, Hebei University, Key Laboratory 
      of Analytical Science and Technology, Hebei Province, Key Laboratory of Chemical 
      Biology, Hebei Province, Baoding 071002, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Ammonium Compounds)
RN  - 6FO62043WK (Glycyrrhizic Acid)
SB  - IM
MH  - Ammonium Compounds/chemistry
MH  - *Chromatography, High Pressure Liquid
MH  - *Chromatography, Reverse-Phase
MH  - Glycyrrhizic Acid/*chemistry
EDAT- 2014/01/08 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/01/08 06:00
PHST- 2014/01/08 06:00 [entrez]
PHST- 2014/01/08 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.3724/sp.j.1123.2013.03004 [doi]
PST - ppublish
SO  - Se Pu. 2013 Sep;31(9):869-74. doi: 10.3724/sp.j.1123.2013.03004.