PMID- 24392651
OWN - NLM
STAT- MEDLINE
DCOM- 20150130
LR  - 20181202
IS  - 2042-7158 (Electronic)
IS  - 0022-3573 (Linking)
VI  - 66
IP  - 6
DP  - 2014 Jun
TI  - Protective effect of tomatine against hydrogen peroxide-induced neurotoxicity in 
      neuroblastoma (SH-SY5Y) cells.
PG  - 844-54
LID - 10.1111/jphp.12205 [doi]
AB  - OBJECTIVES: Reactive oxygen species can induce cell apoptosis, and oxidative 
      stress has been implicated in a variety of neurodegenerative disorders. Tomatine, 
      which is a naturally occurring steroidal glycoalkaloid isolated from Solanum 
      cathayanum, has shown potent anti-oxidant properties. METHODS: In this study, we 
      used the SH-SY5Y cell line as an in vitro model and investigated the protective 
      effect of tomatine against hydrogen peroxide (H2 O2 )-induced neurotoxicity in 
      SH-SY5Y cells. KEY FINDINGS: Tomatine might inhibit the release of cellular 
      lactate dehydrogenase, increase anti-oxidant enzyme activity and glutathione 
      content, reverse the downregulated protein expression of the brain-derived 
      neurotrophic factor (BDNF), inhibit expression of Bax and activations of 
      caspase-3 and caspase-9 in H2 O2 -induced SH-SY5Y cells. CONCLUSIONS: Tomatine 
      exerted beneficially neuroprotective effect on H2 O2 -induced SH-SY5Y cells, 
      mainly enhancing intracellular anti-oxidant enzyme activity and BDNF expression, 
      inhibiting H2 O2 -induced oxidative stress as well as expression of Bax and 
      activations of caspase-3 and caspase-9, alleviating H2 O2 -induced SH-SY5Y cell 
      injury and cell death.
CI  - (c) 2014 Royal Pharmaceutical Society.
FAU - Huang, Shao-lan
AU  - Huang SL
AD  - Hubei Key Laboratory of Natural Products Research and Development, College of 
      Chemistry and Life Science, China Three Gorges University, Yichang, China.
FAU - He, Hai-bo
AU  - He HB
FAU - Zou, Kun
AU  - Zou K
FAU - Bai, Cai-hong
AU  - Bai CH
FAU - Xue, Yan-hong
AU  - Xue YH
FAU - Wang, Jun-zhi
AU  - Wang JZ
FAU - Chen, Jian-feng
AU  - Chen JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140107
PL  - England
TA  - J Pharm Pharmacol
JT  - The Journal of pharmacy and pharmacology
JID - 0376363
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuroprotective Agents)
RN  - 31U6547O08 (Tomatine)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 9)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/analysis
MH  - Caspase 3/metabolism
MH  - Caspase 9/metabolism
MH  - Cell Line, Tumor
MH  - Humans
MH  - Hydrogen Peroxide/*toxicity
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Neuroblastoma/pathology
MH  - Neuroprotective Agents/*pharmacology
MH  - Tomatine/*pharmacology
OTO - NOTNLM
OT  - SH-SY5Y cell
OT  - apoptosis
OT  - neurotoxicity
OT  - reactive oxygen species
OT  - tomatine
EDAT- 2014/01/08 06:00
MHDA- 2015/01/31 06:00
CRDT- 2014/01/08 06:00
PHST- 2013/08/15 00:00 [received]
PHST- 2013/11/30 00:00 [accepted]
PHST- 2014/01/08 06:00 [entrez]
PHST- 2014/01/08 06:00 [pubmed]
PHST- 2015/01/31 06:00 [medline]
AID - 10.1111/jphp.12205 [doi]
PST - ppublish
SO  - J Pharm Pharmacol. 2014 Jun;66(6):844-54. doi: 10.1111/jphp.12205. Epub 2014 Jan 
      7.