PMID- 24393634
OWN - NLM
STAT- MEDLINE
DCOM- 20140509
LR  - 20220408
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 168
IP  - 2-4
DP  - 2014 Jan 31
TI  - Porcine reproductive and respiratory syndrome virus (PRRSV) surveillance using 
      pre-weaning oral fluid samples detects circulation of wild-type PRRSV.
PG  - 331-9
LID - S0378-1135(13)00561-0 [pii]
LID - 10.1016/j.vetmic.2013.11.035 [doi]
AB  - Oral fluid samples collected from litters of piglets (n=600) one day prior to 
      weaning were evaluated as a method to surveil for porcine reproductive and 
      respiratory syndrome virus (PRRSV) infections in four sow herds of approximately 
      12,500 sow each. Serum samples from the litters' dam (n=600) were included for 
      comparison. All four herds were endemically infected with PRRSV and all sows had 
      been vaccinated >/= 2 times with PRRSV modified-live virus vaccines. After all 
      specimens had been collected, samples were randomized and assayed by PRRSV 
      real-time reverse transcription polymerase chain reaction (RT-qPCR) and four 
      PRRSV antibody ELISA assays (IgM, IgA, IgG, and Commercial Kit). All sow serum 
      samples were negative by PRRSV RT-qPCR, but 9 of 600 oral fluid samples tested 
      positive at two laboratories. Open reading frame 5 (ORF5) sequencing of 2 of the 
      9 positive oral fluid samples identified wild-type viruses as the source of the 
      infection. A comparison of antibody responses in RT-qPCR positive vs. negative 
      oral fluid samples showed significantly higher IgG S/P ratios in RT-qPCR-positive 
      oral fluid samples (mean S/P 3.46 vs. 2.36; p=0.02). Likewise, sow serum samples 
      from RT-qPCR-positive litter oral fluid samples showed significantly higher serum 
      IgG (mean S/P 1.73 vs. 0.98; p<0.001) and Commercial Kit (mean S/P 1.97 vs. 0.98; 
      p<0.001) S/P ratios. Overall, the study showed that pre-weaning litter oral fluid 
      samples could provide an efficient and sensitive approach to surveil for PRRSV in 
      infected, vaccinated, or presumed-negative pig breeding herds.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Kittawornrat, Apisit
AU  - Kittawornrat A
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
FAU - Panyasing, Yaowalak
AU  - Panyasing Y
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA; Department of 
      Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 
      Thailand.
FAU - Goodell, Christa
AU  - Goodell C
AD  - IDEXX Laboratories, Inc., Westbrook, ME 04092, USA.
FAU - Wang, Chong
AU  - Wang C
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA; Department of 
      Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, IA 
      50011, USA.
FAU - Gauger, Phillip
AU  - Gauger P
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
FAU - Harmon, Karen
AU  - Harmon K
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
FAU - Rauh, Rolf
AU  - Rauh R
AD  - Tetracore((R)), Inc., Rockville, MD 20850, USA.
FAU - Desfresne, Luc
AU  - Desfresne L
AD  - Seaboard Farms, Inc., Guymon, OK 73942, USA.
FAU - Levis, Ian
AU  - Levis I
AD  - Seaboard Farms, Inc., Guymon, OK 73942, USA.
FAU - Zimmerman, Jeffrey
AU  - Zimmerman J
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. Electronic 
      address: jjzimm@iastate.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131214
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Vaccines, Attenuated)
SB  - IM
MH  - Animals
MH  - Animals, Suckling/virology
MH  - Antibodies, Viral/analysis/blood
MH  - DNA, Viral/genetics
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - Genotype
MH  - Immunoglobulin A/analysis
MH  - Male
MH  - Open Reading Frames
MH  - Population Surveillance/methods
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/*epidemiology/prevention 
      & control
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/*isolation & purification
MH  - Saliva/chemistry/*virology
MH  - Seroepidemiologic Studies
MH  - Sus scrofa/virology
MH  - Swine/virology
MH  - Vaccines, Attenuated/administration & dosage
OTO - NOTNLM
OT  - Oral fluid antibody
OT  - PRRSV
OT  - RT-qPCR
OT  - Surveillance
EDAT- 2014/01/08 06:00
MHDA- 2014/05/10 06:00
CRDT- 2014/01/08 06:00
PHST- 2013/07/10 00:00 [received]
PHST- 2013/11/16 00:00 [revised]
PHST- 2013/11/25 00:00 [accepted]
PHST- 2014/01/08 06:00 [entrez]
PHST- 2014/01/08 06:00 [pubmed]
PHST- 2014/05/10 06:00 [medline]
AID - S0378-1135(13)00561-0 [pii]
AID - 10.1016/j.vetmic.2013.11.035 [doi]
PST - ppublish
SO  - Vet Microbiol. 2014 Jan 31;168(2-4):331-9. doi: 10.1016/j.vetmic.2013.11.035. 
      Epub 2013 Dec 14.