PMID- 24393874
OWN - NLM
STAT- MEDLINE
DCOM- 20140310
LR  - 20161128
IS  - 0385-0684 (Print)
IS  - 0385-0684 (Linking)
VI  - 40
IP  - 12
DP  - 2013 Nov
TI  - [Photodynamic diagnosis and preoperative chemotherapy for biliary tract cancer].
PG  - 1641-3
AB  - BACKGROUND: Cancer cells synthesize substantial amounts of protoporphyrin IX( 
      PPIX) from aminolevulinic acid( ALA). PPIX emits red fluorescence when 
      illuminated under blue light. Photodynamic diagnosis (PDD), based on this 
      phenomenon, is currently used; however, various microorganisms also show the same 
      fluorescence with ALA when illuminated under blue light, resulting in 
      false-positive PDD results. PURPOSE AND METHODS: To avoid misdiagnosis, we 
      incorporated novel systems into the PDD system. ALA, blue light (wavelength, 
      380-450 nm), different kinds of cell lines, and bacteria were used in this in 
      vitro study. We used a 70% deacetylated chitosan solution (DAC-70 Sol), developed 
      in-house, as an antibacterial agent and prepared ALA/DAC-70 Sol, used as a novel 
      photoimaging agent. The antibacterial function of ALA/DAC-70 Sol was examined in 
      vitro, and the photodiagnostic effects on using the novel systems were clinically 
      evaluated using bile from patients with biliary tract cancer. RESULTS: DAC-70 Sol 
      demonstrated an effective bactericidal function in vitro. Red fluorescence could 
      clearly be identified, enabling the detection of cancer cells in the bile using 
      ALA/DAC-70 Sol. CONCLUSIONS: Our novel systems have a great potential for use in 
      clinical photodynamic cytodiagnosis( PDCD), which plays an important role in 
      preoperative cancer chemotherapy.
FAU - Ito, Naoko
AU  - Ito N
AD  - Dept. of Surgery, School of Medicine, Iwate Medical University.
FAU - Sugitachi, Akio
AU  - Sugitachi A
FAU - Takahashi, Masahiro
AU  - Takahashi M
FAU - Makabe, Kenji
AU  - Makabe K
FAU - Kanno, Shoji
AU  - Kanno S
FAU - Hasegawa, Yasushi
AU  - Hasegawa Y
FAU - Takahara, Takeshi
AU  - Takahara T
FAU - Fujita, Tomohiro
AU  - Fujita T
FAU - Nishizuka, Satoshi
AU  - Nishizuka S
FAU - Nitta, Hiroyuki
AU  - Nitta H
FAU - Wakabayashi, Go
AU  - Wakabayashi G
LA  - jpn
PT  - Journal Article
PL  - Japan
TA  - Gan To Kagaku Ryoho
JT  - Gan to kagaku ryoho. Cancer & chemotherapy
JID - 7810034
RN  - 0 (Protoporphyrins)
RN  - 88755TAZ87 (Aminolevulinic Acid)
RN  - C2K325S808 (protoporphyrin IX)
SB  - IM
MH  - Aminolevulinic Acid/metabolism
MH  - Biliary Tract Neoplasms/chemistry/*pathology/ultrastructure
MH  - Cell Line, Tumor
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - *Photochemical Processes
MH  - Protoporphyrins/*analysis/metabolism
MH  - Spectrometry, Fluorescence/*methods
EDAT- 2014/01/08 06:00
MHDA- 2014/03/13 06:00
CRDT- 2014/01/08 06:00
PHST- 2014/01/08 06:00 [entrez]
PHST- 2014/01/08 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
PST - ppublish
SO  - Gan To Kagaku Ryoho. 2013 Nov;40(12):1641-3.