PMID- 24394488
OWN - NLM
STAT- MEDLINE
DCOM- 20141028
LR  - 20151119
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 65
DP  - 2014 Mar
TI  - In vitro effect of deoxynivalenol (DON) mycotoxin on porcine reproductive and 
      respiratory syndrome virus replication.
PG  - 219-26
LID - S0278-6915(13)00865-X [pii]
LID - 10.1016/j.fct.2013.12.043 [doi]
AB  - Deoxynivalenol (DON) is a mycotoxin produced by Fusarium spp. Among monogastric 
      farm animals, swine are the most susceptible to DON as it markedly reduces feed 
      intake and decreases weight gain. DON has also been shown to increase 
      susceptibility to viral infections; therefore the objective of this study was to 
      investigate in vitro impact of DON on porcine reproductive and respiratory 
      syndrome virus (PRRSV). Permissive cells were infected or not with PRRSV and were 
      treated with increasing concentrations of DON. Cell survival and mortality were 
      evaluated by determining the number of viable cells with a tetrazolium compound 
      and by measuring lactate dehydrogenase (LDH) release, respectively. Virus 
      titration and antiviral cytokines mRNA expression were evaluated by quantitative 
      PCR. DON significantly affected the survival of noninfected cells in a dose 
      dependent manner. However, DON concentrations between 140 and 280 significantly 
      increased the survival of cells infected with PRRSV. These concentrations 
      significantly decreased PRRSV replication by inducing a pro-inflammatory 
      cytokines environment and an early activation of apoptosis, which in turn seem to 
      interrupt viral replication. For the first time, this study showed that DON had 
      significant effects on the survival of PRRSV infected cells and on virus 
      replication, in a dose dependent manner.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Savard, Christian
AU  - Savard C
AD  - Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Centre de 
      Recherche en Infectiologie Porcine (CRIP), Saint-Hyacinthe, Quebec, Canada.
FAU - Pinilla, Vicente
AU  - Pinilla V
AD  - Departement de Biomedecine Veterinaire, Saint-Hyacinthe, Quebec, Canada.
FAU - Provost, Chantale
AU  - Provost C
AD  - Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Centre de 
      Recherche en Infectiologie Porcine (CRIP), Saint-Hyacinthe, Quebec, Canada.
FAU - Segura, Mariela
AU  - Segura M
AD  - Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Centre de 
      Recherche en Infectiologie Porcine (CRIP), Saint-Hyacinthe, Quebec, Canada.
FAU - Gagnon, Carl A
AU  - Gagnon CA
AD  - Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Centre de 
      Recherche en Infectiologie Porcine (CRIP), Saint-Hyacinthe, Quebec, Canada.
FAU - Chorfi, Younes
AU  - Chorfi Y
AD  - Departement de Biomedecine Veterinaire, Saint-Hyacinthe, Quebec, Canada. 
      Electronic address: younes.chorfi@umontreal.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140103
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (DNA Primers)
RN  - 0 (Trichothecenes)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - JT37HYP23V (deoxynivalenol)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - DNA Primers
MH  - In Vitro Techniques
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Polymerase Chain Reaction
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects/physiology
MH  - Swine
MH  - Trichothecenes/*pharmacology
MH  - Virus Replication/*drug effects
OTO - NOTNLM
OT  - Deoxynivalenol
OT  - In vitro
OT  - PRRSV
OT  - Swine
OT  - Virus replication
EDAT- 2014/01/08 06:00
MHDA- 2014/10/29 06:00
CRDT- 2014/01/08 06:00
PHST- 2013/07/29 00:00 [received]
PHST- 2013/12/23 00:00 [revised]
PHST- 2013/12/27 00:00 [accepted]
PHST- 2014/01/08 06:00 [entrez]
PHST- 2014/01/08 06:00 [pubmed]
PHST- 2014/10/29 06:00 [medline]
AID - S0278-6915(13)00865-X [pii]
AID - 10.1016/j.fct.2013.12.043 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2014 Mar;65:219-26. doi: 10.1016/j.fct.2013.12.043. Epub 2014 
      Jan 3.