PMID- 24396400
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 7
IP  - 2
DP  - 2014 Feb
TI  - Effects of different types of fluid resuscitation on hepatic mitochondria and 
      apoptosis.
PG  - 335-342
AB  - The aim of this study was to observe the effects of different types of fluid 
      resuscitation on hepatic mitochondria and apoptosis in hemorrhagic shock, and the 
      corresponding mechanisms. Forty rats were divided into five groups: Sham surgery 
      (Sham group), shock (Shock group), Ringer's lactate resuscitation (RL group), 
      hydroxyethyl starch resuscitation (HES group) and autologous blood resuscitation 
      (BL group). A model of hemorrhagic shock was successfully induced in the latter 
      four groups. The recovery objective was to maintain the mean arterial pressure 
      (MAP) of the rats at 80 mmHg. Two hours after the end of the recovery experiment, 
      fresh liver samples were examined in order to observe the changes in the 
      morphology and mitochondrial membrane potential (DeltaPsim). In addition, the levels of 
      succinate dehydrogenase (SDH) activity were assessed, and a terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was 
      conducted to evaluate the level of apoptosis in the liver cells. In the Shock, 
      RL, HES and BL groups, mitochondrial ultrastructural damage in the liver cells, 
      significant reductions in liver cell function, liver DeltaPsim and SDH activity, and 
      the apoptosis of hepatocytes were more apparent compared with those in the Sham 
      group. In the BL group, compared with the RL and HES groups, the injuries to the 
      mitochondrial ultrastructure and liver cell function were significantly reduced, 
      the hepatic DeltaPsim and SDH activity were significantly increased and the hepatocyte 
      apoptosis index (AI) was significantly reduced (P<0.05). In conclusion, in a rat 
      model of hemorrhagic shock, different methods of fluid resuscitation may improve 
      the liver cells with regard to mitochondrial ultrastructure and function, the 
      stability of liver DeltaPsim, the activity of SDH and the inhibition of liver cell 
      apoptosis. The results indicate that infusion with autologous blood followed by 
      RL solution is a preferable method of fluid resuscitation compared with HES.
FAU - Cheng, Qinghong
AU  - Cheng Q
AD  - Department of Intensive Care Unit, First Affiliated Hospital, Medical College, 
      Shihezi University, Shihezi, Xinjiang 832002, P.R. China.
FAU - Yang, Guangtian
AU  - Yang G
AD  - Department of Emergency Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
FAU - Ma, Juanzhen
AU  - Ma J
AD  - Department of Intensive Care Unit, First Affiliated Hospital, Medical College, 
      Shihezi University, Shihezi, Xinjiang 832002, P.R. China.
FAU - Li, Jianhua
AU  - Li J
AD  - Department of Intensive Care Unit, First Affiliated Hospital, Medical College, 
      Shihezi University, Shihezi, Xinjiang 832002, P.R. China.
FAU - Shan, Qi
AU  - Shan Q
AD  - Department of Intensive Care Unit, First Affiliated Hospital, Medical College, 
      Shihezi University, Shihezi, Xinjiang 832002, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20131212
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC3881054
OTO - NOTNLM
OT  - apoptosis
OT  - fluid resuscitation
OT  - hemorrhagic shock
OT  - mitochondrial membrane potential
OT  - succinate dehydrogenase
EDAT- 2014/01/08 06:00
MHDA- 2014/01/08 06:01
PMCR- 2013/12/12
CRDT- 2014/01/08 06:00
PHST- 2013/06/18 00:00 [received]
PHST- 2013/10/24 00:00 [accepted]
PHST- 2014/01/08 06:00 [entrez]
PHST- 2014/01/08 06:00 [pubmed]
PHST- 2014/01/08 06:01 [medline]
PHST- 2013/12/12 00:00 [pmc-release]
AID - etm-07-02-0335 [pii]
AID - 10.3892/etm.2013.1447 [doi]
PST - ppublish
SO  - Exp Ther Med. 2014 Feb;7(2):335-342. doi: 10.3892/etm.2013.1447. Epub 2013 Dec 
      12.