PMID- 24396440
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 7
IP  - 2
DP  - 2014 Feb
TI  - Diagnosis of bladder cancer from the voided urine specimens using multi-target 
      fluorescence in situ hybridization.
PG  - 325-330
AB  - The present study aimed to evaluate the diagnostic value of chromosomal analysis 
      by fluorescence in situ hybridization (FISH) for bladder cancer in light of the 
      histological diagnosis. Several valuable observations using FISH technologies in 
      voided urine cells were also reported. The multi-target FISH-containing probes 
      for the centromeres of chromosomes 3, 7 and 17 and the 9p21 locus were applied to 
      cytospin specimens prepared from voided urine. Urine samples from 53 bladder 
      cancer patients and 30 patients with benign alterations were used for this study. 
      The histological observations of surgical resection specimens showed that the 
      specificity and sensitivity for the technique were 100.0 and 88.0%, respectively. 
      Statistical analyses showed that there was no significant correlation between 
      FISH-positive rate and the tumor stage/grade (P<0.05). However, the proportion of 
      tumor cells with genetic abnormalities positively correlated with the tumor stage 
      (P<0.01). Furthermore, the number of abnormal cells in muscle-invasive pT2 was 
      significantly higher than that in non-muscle-invasive pTa, pT1 (P<0.01). Of 50 
      patients with bladder cancer, polysomies of chromosomes 3, 7 and 17 were detected 
      in 84.0, 48.0 and 78.0% of cases, respectively, and loss of the 9p21 gene was 
      detected in 80.0% of cases. In addition, the detailed results from different 
      urine specimens showed that FISH assay was required. FISH assay for chromosomes 
      3, 7 and 17 and 9p21 has a high specificity and sensitivity in the detection of 
      bladder cancer and may reduce the necessity for cytoscopy treatment.
FAU - Ke, Zunfu
AU  - Ke Z
AD  - Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Lai, Yuanhua
AU  - Lai Y
AD  - Department of Vascular and Thyroid Surgery, The First Affiliated Hospital of Sun 
      Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
FAU - Ma, Xudong
AU  - Ma X
AD  - Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, Fujian 
      363000, P.R. China.
FAU - Lil, Shuhua
AU  - Lil S
AD  - Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Huang, Wenhua
AU  - Huang W
AD  - Department of Anatomy, School of Basic Medical Science, Southern Medical 
      University, Guangzhou, Guangdong 510515, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20131209
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC3881196
OTO - NOTNLM
OT  - bladder cancer
OT  - fluorescence in situ hybridization
OT  - molecular pathology
EDAT- 2014/01/08 06:00
MHDA- 2014/01/08 06:01
PMCR- 2013/12/09
CRDT- 2014/01/08 06:00
PHST- 2013/06/07 00:00 [received]
PHST- 2013/11/12 00:00 [accepted]
PHST- 2014/01/08 06:00 [entrez]
PHST- 2014/01/08 06:00 [pubmed]
PHST- 2014/01/08 06:01 [medline]
PHST- 2013/12/09 00:00 [pmc-release]
AID - ol-07-02-0325 [pii]
AID - 10.3892/ol.2013.1744 [doi]
PST - ppublish
SO  - Oncol Lett. 2014 Feb;7(2):325-330. doi: 10.3892/ol.2013.1744. Epub 2013 Dec 9.