PMID- 24398378
OWN - NLM
STAT- MEDLINE
DCOM- 20140815
LR  - 20211021
IS  - 1759-6653 (Electronic)
IS  - 1759-6653 (Linking)
VI  - 6
IP  - 1
DP  - 2014 Jan
TI  - Insights into the preservation of the homomorphic sex-determining chromosome of 
      Aedes aegypti from the discovery of a male-biased gene tightly linked to the 
      M-locus.
PG  - 179-91
LID - 10.1093/gbe/evu002 [doi]
AB  - The preservation of a homomorphic sex-determining chromosome in some organisms 
      without transformation into a heteromorphic sex chromosome is a long-standing 
      enigma in evolutionary biology. A dominant sex-determining locus (or M-locus) in 
      an undifferentiated homomorphic chromosome confers the male phenotype in the 
      yellow fever mosquito Aedes aegypti. Genetic evidence suggests that the M-locus 
      is in a nonrecombining region. However, the molecular nature of the M-locus has 
      not been characterized. Using a recently developed approach based on Illumina 
      sequencing of male and female genomic DNA, we identified a novel gene, myo-sex, 
      that is present almost exclusively in the male genome but can sporadically be 
      found in the female genome due to recombination. For simplicity, we define 
      sequences that are primarily found in the male genome as male-biased. 
      Fluorescence in situ hybridization (FISH) on A. aegypti chromosomes demonstrated 
      that the myo-sex probe localized to region 1q21, the established location of the 
      M-locus. Myo-sex is a duplicated myosin heavy chain gene that is highly expressed 
      in the pupa and adult male. Myo-sex shares 83% nucleotide identity and 97% amino 
      acid identity with its closest autosomal paralog, consistent with ancient 
      duplication followed by strong purifying selection. Compared with males, myo-sex 
      is expressed at very low levels in the females that acquired it, indicating that 
      myo-sex may be sexually antagonistic. This study establishes a framework to 
      discover male-biased sequences within a homomorphic sex-determining chromosome 
      and offers new insights into the evolutionary forces that have impeded the 
      expansion of the nonrecombining M-locus in A. aegypti.
FAU - Hall, Andrew Brantley
AU  - Hall AB
AD  - Department of Biochemistry, Virginia Tech.
FAU - Timoshevskiy, Vladimir A
AU  - Timoshevskiy VA
FAU - Sharakhova, Maria V
AU  - Sharakhova MV
FAU - Jiang, Xiaofang
AU  - Jiang X
FAU - Basu, Sanjay
AU  - Basu S
FAU - Anderson, Michelle A E
AU  - Anderson MA
FAU - Hu, Wanqi
AU  - Hu W
FAU - Sharakhov, Igor V
AU  - Sharakhov IV
FAU - Adelman, Zach N
AU  - Adelman ZN
FAU - Tu, Zhijian
AU  - Tu Z
LA  - eng
SI  - GENBANK/KF150020
SI  - SRA/SRP023515
GR  - R56 AI077680/AI/NIAID NIH HHS/United States
GR  - AI 77680/AI/NIAID NIH HHS/United States
GR  - R21 AI088035/AI/NIAID NIH HHS/United States
GR  - R01 AI077680/AI/NIAID NIH HHS/United States
GR  - R21 AI094289/AI/NIAID NIH HHS/United States
GR  - AI88035/AI/NIAID NIH HHS/United States
GR  - AI094289/AI/NIAID NIH HHS/United States
GR  - R21 AI105575/AI/NIAID NIH HHS/United States
GR  - AI105575/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Genome Biol Evol
JT  - Genome biology and evolution
JID - 101509707
RN  - 0 (Insect Proteins)
SB  - IM
MH  - Aedes/*genetics/growth & development/metabolism/physiology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosomes, Insect/*genetics
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - *Genes, Insect
MH  - *Genetic Linkage
MH  - Genetic Loci
MH  - Insect Proteins/genetics/metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - Recombination, Genetic
MH  - *Sex Characteristics
MH  - Sex Chromosomes/*genetics
PMC - PMC3914700
OTO - NOTNLM
OT  - chromosome quotient
OT  - myo-sex
OT  - nonrecombining region
OT  - sex chromosomes
OT  - sex-determination
OT  - sexual antagonism
EDAT- 2014/01/09 06:00
MHDA- 2014/08/16 06:00
PMCR- 2014/01/06
CRDT- 2014/01/09 06:00
PHST- 2014/01/09 06:00 [entrez]
PHST- 2014/01/09 06:00 [pubmed]
PHST- 2014/08/16 06:00 [medline]
PHST- 2014/01/06 00:00 [pmc-release]
AID - evu002 [pii]
AID - 10.1093/gbe/evu002 [doi]
PST - ppublish
SO  - Genome Biol Evol. 2014 Jan;6(1):179-91. doi: 10.1093/gbe/evu002.