PMID- 24400904
OWN - NLM
STAT- MEDLINE
DCOM- 20140901
LR  - 20220310
IS  - 1469-8137 (Electronic)
IS  - 0028-646X (Linking)
VI  - 201
IP  - 3
DP  - 2014 Feb
TI  - The LOSS OF APOMEIOSIS (LOA) locus in Hieracium praealtum can function 
      independently of the associated large-scale repetitive chromosomal structure.
PG  - 973-981
LID - 10.1111/nph.12574 [doi]
AB  - Apomixis or asexual seed formation in Hieracium praealtum (Asteraceae) is 
      controlled by two independent dominant loci. One of these, the LOSS OF APOMEIOSIS 
      (LOA) locus, controls apomixis initiation, mitotic embryo sac formation 
      (apospory) and suppression of the sexual pathway. The LOA locus is found near the 
      end of a hemizygous chromosome surrounded by extensive repeats extending along 
      the chromosome arm. Similar apomixis-carrying chromosome structures have been 
      found in some apomictic grasses, suggesting that the extensive repetitive 
      sequences may be functionally relevant to apomixis. Fluorescence in situ 
      hybridization (FISH) was used to examine chromosomes of apomeiosis deletion 
      mutants and rare recombinants in the critical LOA region arising from a cross 
      between sexual Hieracium pilosella and apomictic H. praealtum. The combined 
      analyses of aposporous and nonaposporous recombinant progeny and chromosomal 
      karyotypes were used to determine that the functional LOA locus can be 
      genetically separated from the very extensive repeat regions found on the 
      LOA-carrying chromosome. The large-scale repetitive sequences associated with the 
      LOA locus in H. praealtum are not essential for apospory or suppression of sexual 
      megasporogenesis (female meiosis).
CI  - (c) 2013 CSIRO. New Phytologist (c) 2013 New Phytologist Trust.
FAU - Kotani, Yoshiko
AU  - Kotani Y
AD  - Laboratory of Plant Molecular Genetics, Division of Natural Science, Osaka Kyoiku 
      University, Kashiwara, Osaka, 582-8582, Japan.
FAU - Henderson, Steven T
AU  - Henderson ST
AD  - Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant 
      Industry, Waite Campus, Hartley Grove, Urrbrae, Adelaide, SA, 5064, Australia.
FAU - Suzuki, Go
AU  - Suzuki G
AD  - Laboratory of Plant Molecular Genetics, Division of Natural Science, Osaka Kyoiku 
      University, Kashiwara, Osaka, 582-8582, Japan.
FAU - Johnson, Susan D
AU  - Johnson SD
AD  - Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant 
      Industry, Waite Campus, Hartley Grove, Urrbrae, Adelaide, SA, 5064, Australia.
FAU - Okada, Takashi
AU  - Okada T
AD  - Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant 
      Industry, Waite Campus, Hartley Grove, Urrbrae, Adelaide, SA, 5064, Australia.
FAU - Siddons, Hayley
AU  - Siddons H
AD  - Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant 
      Industry, Waite Campus, Hartley Grove, Urrbrae, Adelaide, SA, 5064, Australia.
FAU - Mukai, Yasuhiko
AU  - Mukai Y
AD  - Laboratory of Plant Molecular Genetics, Division of Natural Science, Osaka Kyoiku 
      University, Kashiwara, Osaka, 582-8582, Japan.
FAU - Koltunow, Anna M G
AU  - Koltunow AMG
AD  - Commonwealth Scientific and Industrial Research Organization (CSIRO), Plant 
      Industry, Waite Campus, Hartley Grove, Urrbrae, Adelaide, SA, 5064, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131101
PL  - England
TA  - New Phytol
JT  - The New phytologist
JID - 9882884
SB  - IM
MH  - Asteraceae/cytology/*genetics/physiology
MH  - Chromosomes, Plant/*genetics
MH  - Genetic Loci/*genetics
MH  - Genome, Plant/genetics
MH  - Metaphase/genetics
MH  - Physical Chromosome Mapping
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Reproduction/genetics
MH  - Sequence Deletion
OTO - NOTNLM
OT  - Hieracium
OT  - apomeiosis
OT  - apomixis
OT  - apospory
OT  - chromosome structure
OT  - fluorescence in situ hybridization (FISH)
EDAT- 2014/01/10 06:00
MHDA- 2014/09/02 06:00
CRDT- 2014/01/10 06:00
PHST- 2013/08/12 00:00 [received]
PHST- 2013/09/26 00:00 [accepted]
PHST- 2014/01/10 06:00 [entrez]
PHST- 2014/01/10 06:00 [pubmed]
PHST- 2014/09/02 06:00 [medline]
AID - 10.1111/nph.12574 [doi]
PST - ppublish
SO  - New Phytol. 2014 Feb;201(3):973-981. doi: 10.1111/nph.12574. Epub 2013 Nov 1.