PMID- 24401430
OWN - NLM
STAT- MEDLINE
DCOM- 20140902
LR  - 20140109
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 119
DP  - 2014 Feb
TI  - Direct analysis of eight chlorophenols in urine by large volume injection online 
      turbulent flow solid-phase extraction liquid chromatography with multiple 
      wavelength ultraviolet detection.
PG  - 396-400
LID - S0039-9140(13)00880-1 [pii]
LID - 10.1016/j.talanta.2013.11.006 [doi]
AB  - A novel method for determining eight chlorophenols (CPs) by large volume 
      injection online turbulent flow solid-phase extraction high performance liquid 
      chromatography in urine samples was developed. An aliquot of 1.0 mL urine sample 
      could be analyzed directly after centrifugation. The analytes were 
      preconcentrated online on a Turboflow C18-P SPE column, eluted in back-flush 
      mode, and then separated on an Acclaim PA2 analytical column. Major parameters 
      such as SPE column type, sample loading flow rate and elution time were optimized 
      in detail. Eight CPs from monochlorophenol to pentacholophenol were measured by 
      multiple-wavelength UV detection at four different wavelengths. The limits of 
      detection (LODs) were between 0.5 and 2 ng/mL. The linearity range was from the 
      limit of quantification to 1000 ng/mL for each compound, with the coefficients of 
      determination (r(2)) ranging from 0.9990 to 0.9996. The reproducibility of 
      intraday and interday relative standard deviations (RSDs) ranged from 0.6% to 
      4.5% (n=5). The method was successfully applied to analyze eight CPs in urine 
      samples. Good recoveries, ranging from 76.3% to 122.9%, were obtained. This 
      simple, sensitive and accurate method provides an alternative way to rapidly 
      analyze and monitor CPs in urine samples, especially for matters of occupational 
      exposure.
CI  - (c) 2013 Elsevier B.V. All rights reserved.
FAU - Guo, Feng
AU  - Guo F
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
FAU - Liu, Qian
AU  - Liu Q
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
FAU - Shi, Jian-bo
AU  - Shi JB
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China. Electronic address: jbshi@rcees.ac.cn.
FAU - Wei, Fu-sheng
AU  - Wei FS
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China; China National Evironmental Monitoring Center, Beijing, 100012, 
      China.
FAU - Jiang, Gui-bin
AU  - Jiang GB
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131119
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Chlorophenols)
SB  - IM
MH  - Chlorophenols/*urine
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Reproducibility of Results
MH  - Solid Phase Extraction/*methods
MH  - Spectrophotometry, Ultraviolet/*methods
OTO - NOTNLM
OT  - Chlorophenols
OT  - Online solid-phase extraction
OT  - Turbulent flow chromatography
OT  - Urine
EDAT- 2014/01/10 06:00
MHDA- 2014/09/03 06:00
CRDT- 2014/01/10 06:00
PHST- 2013/08/29 00:00 [received]
PHST- 2013/10/23 00:00 [revised]
PHST- 2013/11/01 00:00 [accepted]
PHST- 2014/01/10 06:00 [entrez]
PHST- 2014/01/10 06:00 [pubmed]
PHST- 2014/09/03 06:00 [medline]
AID - S0039-9140(13)00880-1 [pii]
AID - 10.1016/j.talanta.2013.11.006 [doi]
PST - ppublish
SO  - Talanta. 2014 Feb;119:396-400. doi: 10.1016/j.talanta.2013.11.006. Epub 2013 Nov 
      19.