PMID- 24402377
OWN - NLM
STAT- MEDLINE
DCOM- 20150102
LR  - 20211021
IS  - 1434-4726 (Electronic)
IS  - 0937-4477 (Linking)
VI  - 271
IP  - 6
DP  - 2014 Jun
TI  - p63 and Ki-67 immunostainings in laryngeal squamous cell carcinoma are related to 
      survival.
PG  - 1641-51
LID - 10.1007/s00405-013-2833-1 [doi]
AB  - To examine the prognostic significance of the immunohistochemical expression of 
      p63 and Ki-67 oncoproteins in patients with laryngeal squamous cell carcinoma, a 
      retrospective evaluation was carried out on a cohort of 108 patients with primary 
      laryngeal squamous cell carcinoma (LSCC) treated by primary surgery. For the 
      immunohistochemical evaluation, tissue section obtained by formalin-fixed and 
      paraffin-embedded tissue blocks from resection of each patient was used. 
      Clinicopathologic data were associated with the immunostaining results. The 
      association among the considered variables was assessed by Fisher's exact test, 
      Mann-Whitney test, non-parametric chi(2) test, and Spearman's rho rank test was 
      used to assess the relations among them. Differences in p63 and Ki-67 
      immunoreactivity among the different groups were compared via Kruskal-Wallis test 
      and post hoc tests were performed using Mann-Whitney test with Bonferroni 
      correction. The overall survival rate was estimated via Kaplan-Meier method, and 
      the cumulative incidence functions for different groups were compared using 
      log-rank statistics. Cox proportional hazard model was employed in a multivariate 
      analysis to assess the effect of prognostic factors in the overall survival rate. 
      Furthermore, taking into account death due to other causes, we estimated 
      LSCC-related survival and disease-free survival rates using competing risk 
      analysis. The results of immunohistochemical examination showed a statistically 
      significant relationship between the up-regulation of P63 and Ki-67, an increase 
      in histological grading, and primary tumours associated with lymph node 
      metastases. p63 and Ki-67 up-regulation was related to a shorter disease-free 
      survival and a significant association was found between p63 and Ki-67 percentage 
      of positive cells and patient survival. Finally, we noticed a significant 
      relation between p63 and Ki-67 (rho = 0.87). On the other hand, no statistically 
      significant associations were found between p63 and Ki-67 down-regulation and 
      clinicopathologic data. Our findings suggest that abnormal p63 and Ki-67 
      immunoreactivity may be involved in the early phases of laryngeal tumorigenesis 
      and may become a significant prognostic predictor for both overall and 
      disease-free survivals. These biomarkers could thus help in the selection of 
      high-risk patients with LSCC who may benefit from more aggressive therapy or 
      chemoprevention.
FAU - Re, M
AU  - Re M
AD  - Department of Otorhinolaryngology, Polytechnic University of the Marches, 
      "Ospedali Riuniti" of Ancona, Via Conca 71, 60020, Ancona, Italy, 
      remassimo@hotmail.com.
FAU - Zizzi, A
AU  - Zizzi A
FAU - Ferrante, L
AU  - Ferrante L
FAU - Stramazzotti, D
AU  - Stramazzotti D
FAU - Goteri, G
AU  - Goteri G
FAU - Gioacchini, F M
AU  - Gioacchini FM
FAU - Olivieri, F
AU  - Olivieri F
FAU - Magliulo, G
AU  - Magliulo G
FAU - Rubini, C
AU  - Rubini C
LA  - eng
PT  - Journal Article
DEP - 20140109
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (CKAP4 protein, human)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*metabolism/pathology/surgery
MH  - Cohort Studies
MH  - Disease-Free Survival
MH  - Female
MH  - Head and Neck Neoplasms/*metabolism/pathology/surgery
MH  - Humans
MH  - Immunohistochemistry
MH  - Kaplan-Meier Estimate
MH  - Ki-67 Antigen/*metabolism
MH  - Laryngeal Neoplasms/*metabolism/pathology/surgery
MH  - Laryngectomy
MH  - Male
MH  - Membrane Proteins/*metabolism
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Proportional Hazards Models
MH  - Retrospective Studies
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Up-Regulation
EDAT- 2014/01/10 06:00
MHDA- 2015/01/03 06:00
CRDT- 2014/01/10 06:00
PHST- 2013/08/12 00:00 [received]
PHST- 2013/11/19 00:00 [accepted]
PHST- 2014/01/10 06:00 [entrez]
PHST- 2014/01/10 06:00 [pubmed]
PHST- 2015/01/03 06:00 [medline]
AID - 10.1007/s00405-013-2833-1 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2014 Jun;271(6):1641-51. doi: 
      10.1007/s00405-013-2833-1. Epub 2014 Jan 9.