PMID- 24403550
OWN - NLM
STAT- MEDLINE
DCOM- 20141121
LR  - 20140221
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 90
IP  - 2
DP  - 2014 Feb
TI  - Toll-like receptor 11-initiated innate immune response in male mouse germ cells.
PG  - 38
LID - 10.1095/biolreprod.113.114421 [doi]
AB  - Toxoplasma gondii and uropathogenic Escherichia coli (UPEC) may infect the testis 
      and impair testicular function. Mechanisms underlying testicular innate immune 
      response to these two pathogens remain to be clarified. The present study 
      examined the function of TLR11, which can be recognized by T. gondii-derived 
      profilin and UPEC, in initiating innate immune response in male mouse germ cells. 
      TLR11 is predominantly expressed in spermatids. Profilin and UPEC induced the 
      expressions of different inflammatory cytokine profiles in the germ cells. In 
      particular, profilin induced the expressions of macrophage chemotactic protein 1 
      (MCP1), interleukin 12 (IL12), and interferon gamma (IFNG) through nuclear factor 
      KB (NFKB) activation. UPEC induced the expressions of MCP1, IL12, and IFNG, as 
      well as tumor necrosis factor alpha (TNFA), IL6, and IFNB, through the activation 
      of NFKB, IFN regulatory factor 3, and mitogen-activated protein kinases. Evidence 
      showed that profilin induced the innate response in male germ cells through TLR11 
      signaling, and UPEC triggered the response through TLR11 and other TLR-signaling 
      pathways. We also provided evidence that local injection of profilin or UPEC 
      induces the innate immune response in the germ cells. Data describe 
      TLR11-mediated innate immune function of male germ cells in response to T. gondii 
      profilin and UPEC stimulations. This system may play a role in testicular defense 
      against T. gondii and UPEC infections in mice.
FAU - Chen, Qiaoyuan
AU  - Chen Q
AD  - Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences; School 
      of Basic Medicine, Peking Union Medical College, Beijing, China.
FAU - Zhu, Weiwei
AU  - Zhu W
FAU - Liu, Zhenghui
AU  - Liu Z
FAU - Yan, Keqin
AU  - Yan K
FAU - Zhao, Shutao
AU  - Zhao S
FAU - Han, Daishu
AU  - Han D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140220
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Tlr11 protein, mouse)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Escherichia coli Infections/immunology
MH  - Germ Cells/immunology/metabolism
MH  - Immunity, Innate/*genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Spermatozoa/*immunology/metabolism
MH  - Toll-Like Receptors/*physiology
MH  - Toxoplasma/immunology
MH  - Toxoplasmosis/immunology
MH  - Uropathogenic Escherichia coli/immunology
OTO - NOTNLM
OT  - T. gondii
OT  - Toll-like receptor 11
OT  - male mouse germ cell
OT  - uropathogenic E. coli
EDAT- 2014/01/10 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/01/10 06:00
PHST- 2014/01/10 06:00 [entrez]
PHST- 2014/01/10 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - biolreprod.113.114421 [pii]
AID - 10.1095/biolreprod.113.114421 [doi]
PST - epublish
SO  - Biol Reprod. 2014 Feb 20;90(2):38. doi: 10.1095/biolreprod.113.114421. Print 2014 
      Feb.