PMID- 24405418
OWN - NLM
STAT- MEDLINE
DCOM- 20141230
LR  - 20140512
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 23
IP  - 10
DP  - 2014 May 15
TI  - A mouse bone marrow stromal cell line with skeletal stem cell characteristics to 
      study osteogenesis in vitro and in vivo.
PG  - 1097-108
LID - 10.1089/scd.2013.0367 [doi]
AB  - Bone marrow stromal cells (BMSCs) are composed of progenitor and multipotent 
      skeletal stem cells, which are able to differentiate in vitro into osteocytes, 
      adipocytes, and chondrocytes. Mouse BMSCs (mBMSCs) are a versatile model system 
      to investigate factors involved in BMSC differentiation in vitro and in vivo as a 
      variety of transgenic mouse models are available. In this study, mBMSCs were 
      isolated and osteogenic differentiation was investigated in tissue culture and in 
      vivo. Three out of seven independent cell isolates showed the ability to 
      differentiate into osteocytes, adipocytes, and chondrocytes in vitro. In vitro 
      multipotency of an established mBMSC line was maintained over 45 passages. The 
      osteogenic differentiation of this cell line was confirmed by quantitative 
      polymerase chain reaction (qPCR) analysis of specific markers such as osteocalcin 
      and shown to be Runx2 dependent. Notably, the cell line, when transplanted 
      subcutaneously into mice, possesses full skeletal stem cell characteristics in 
      vivo in early and late passages, evident from bone tissue formation, induction of 
      vascularization, and hematopoiesis. This cell line provides, thus, a versatile 
      tool to unravel the molecular mechanisms governing osteogenesis in vivo thereby 
      aiding to improve current strategies in bone regenerative therapy.
FAU - Raeth, Sebastian
AU  - Raeth S
AD  - 1 Institute of Cell Biology and Immunology, University of Stuttgart , Stuttgart, 
      Germany .
FAU - Sacchetti, Benedetto
AU  - Sacchetti B
FAU - Siegel, Georg
AU  - Siegel G
FAU - Mau-Holzmann, Ulrike A
AU  - Mau-Holzmann UA
FAU - Hansmann, Jan
AU  - Hansmann J
FAU - Vacun, Gabriele
AU  - Vacun G
FAU - Hauk, Thomas G
AU  - Hauk TG
FAU - Pfizenmaier, Klaus
AU  - Pfizenmaier K
FAU - Hausser, Angelika
AU  - Hausser A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140218
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Runx2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*metabolism
MH  - *Cell Differentiation
MH  - Core Binding Factor Alpha 1 Subunit/metabolism
MH  - Mice
MH  - Multipotent Stem Cells/cytology/*metabolism
MH  - *Osteogenesis
MH  - Stromal Cells/cytology/metabolism
EDAT- 2014/01/11 06:00
MHDA- 2014/12/31 06:00
CRDT- 2014/01/11 06:00
PHST- 2014/01/11 06:00 [entrez]
PHST- 2014/01/11 06:00 [pubmed]
PHST- 2014/12/31 06:00 [medline]
AID - 10.1089/scd.2013.0367 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2014 May 15;23(10):1097-108. doi: 10.1089/scd.2013.0367. Epub 
      2014 Feb 18.