PMID- 24405535
OWN - NLM
STAT- MEDLINE
DCOM- 20140717
LR  - 20140110
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 93
IP  - 40
DP  - 2013 Oct 29
TI  - [A comparative study of fluorescence in situ hybridization versus conventional 
      cytogenetics in the detection of clonal aberrations in myelodysplastic syndrome].
PG  - 3175-9
AB  - OBJECTIVE: To compare the results of fluorescence in situ hybridization (FISH) 
      versus conventional cytogenetics (CC) in the detection of common chromosomal 
      abnormalities and evaluate the significance of FISH in myelodysplastic syndrome 
      (MDS) . METHODS: A total of 344 patients with de novo MDS from June 2008 to 
      October 2012 were detected by 6 pairs of probes, including CSF1R/D5S23-D5S721 
      (5q33) , EGR1/ D5S23-D5S721 (5q31) , D7S486 (7q31) /CSP7, D7S522 (7q31) /CSP7, 
      D20S108/CSP8 (20q12/CSP8) and CSPX/CSPY. The results were compared with those of 
      CC. RESULTS: CC revealed cytogenetic abnormalities in 168/344 cases (48.8%) and 
      the frequency of common aberrations such as +8, 20q-, -7/7q-, -5/5q- and -Y were 
      18.9% (65/344) , 9.3% (32/344), 8.4% (29/344), 8.4% (29/344) and 2.4% (5/206) 
      respectively. While FISH revealed chromosome abnormalities in 147/344 patients 
      (42.7%) and the frequency of +8, 20q-, -7/7q-, -5/5q- and -Y were 20.9% (72/344), 
      11.6% (40/344), 11.6% (40/344), 10.2% (35/344) and 2.9% (6/206) respectively. 
      Overall 187/344 patients (54.4%) carried clonal aberrations by a combination of 
      CC and FISH. Among 158 patients with normal karyotype by CC, 14 cases (8.9%) were 
      detected to have clonal aberrations by FISH. FISH also confirmed 4 carriers of 
      clonal aberrations out of 9 patients with non clonal abnormalities by CC. 
      CONCLUSIONS: FISH is effective for improving the probability of detecting 
      chromosome abnormalities in MDS cases with normal karyotypes and karyotype 
      failure. FISH may provide rationales for clonal abnormalities in patients with 
      non clonal aberrations by CC. A combination of FISH and CC shows complementary 
      advantages.
FAU - Lai, Yue-yun
AU  - Lai YY
AD  - Peking University People's Hospital, Peking University Institute of Hematology, 
      Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 
      100044, China.
FAU - Li, Na
AU  - Li N
FAU - Feng, Lin
AU  - Feng L
FAU - Shi, Yan
AU  - Shi Y
FAU - Dang, Hui
AU  - Dang H
FAU - He, Qi
AU  - He Q
FAU - Wang, Zheng
AU  - Wang Z
FAU - Wang, Xiao-yan
AU  - Wang XY
FAU - Li, Ye
AU  - Li Y
FAU - Liu, Qing
AU  - Liu Q
FAU - Huang, Xiao-jun
AU  - Huang XJ
AD  - Peking University People's Hospital, Peking University Institute of Hematology, 
      Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 
      100044, China; Peking-Tsinghua Center for Life Sciences, Beijing 100871, China. 
      Email: xjhrm@medmail.com.
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Child, Preschool
MH  - Cytogenetics/*methods
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/diagnosis/*genetics
MH  - Young Adult
EDAT- 2014/01/11 06:00
MHDA- 2014/07/18 06:00
CRDT- 2014/01/11 06:00
PHST- 2014/01/11 06:00 [entrez]
PHST- 2014/01/11 06:00 [pubmed]
PHST- 2014/07/18 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2013 Oct 29;93(40):3175-9.