PMID- 24405593
OWN - NLM
STAT- MEDLINE
DCOM- 20140711
LR  - 20181203
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 30
IP  - 1
DP  - 2014 Jan
TI  - [Bone marrow-derived mesenchymal stem cells from estrogen deficiency induced 
      osteoporosis mice regulate T cell migration and apoptosis through expressing 
      MCP-1].
PG  - 19-22
AB  - OBJECTIVE: To reveal the role of bone marrow-derived mesenchymal stem cells 
      (BMSCs) in the development of osteoporosis by comparing the differences in 
      monocyte chemoattractant protein-1 (MCP-1) expression and T cells' migration and 
      apoptosis induced by BMSCs from ovariectomy (OVX) group and sham group. METHODS: 
      OVX was performed on C57BL/6 mice to establish the animal models of osteoporosis. 
      Osteoporosis was confirmed by micro-CT. The expression of MCP-1 between OVX group 
      and sham group was examined by ELISA; after exogenous estrogen of different 
      concentrations were given to stimulate BMSCs from OVX group, the expression of 
      MCP-1 was observed again by ELISA. Through co-culturing of BMSCs and T cells, the 
      change of T cells' migration and apoptosis capacity induced by BMSCs was compared 
      between OVX group and sham group. And also, we observed the effects of exogenous 
      estrogen of different concentrations on the T cells' migration and apoptosis 
      capacity. RESULTS: In animal models of osteoporosis induced by estrogen 
      deficiency, BMSCs had a declined inducing effect on the capacity of T cell 
      migration and apoptosis and expressed a decreased level of migration-related gene 
      MCP-1. After the stimulation of estrogen of certain concentration, the declining 
      tendency was revised to some extent. CONCLUSION: Through expressing MCP-1, BMSCs 
      could regulate the capacity of T cell migration and apoptosis, thus leading to 
      the development of osteoporosis.
FAU - Yu, Yang
AU  - Yu Y
AD  - Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical 
      University; Chongqing Key Laboratory of Oral Diseases and Biomedical Science, 
      Chongqing 400015, China.
FAU - Shao, Bingyi
AU  - Shao B
AD  - Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical 
      University; Chongqing Key Laboratory of Oral Diseases and Biomedical Science, 
      Chongqing 400015, China.
FAU - Shang, Fengqing
AU  - Shang F
AD  - Department of Orthodontics, School of Stomatology, Fourth Military Medical 
      University, Xi'an 710032, China.
FAU - Zhou, Zhifei
AU  - Zhou Z
AD  - Department of Pediatric Dentistry, Affiliated Hospital of Stomatology, School of 
      Stomatology, Fourth Military Medical University, Xi'an 710032, China.
FAU - Wang, Xi
AU  - Wang X
AD  - Department of Orthodontics, School of Stomatology, Fourth Military Medical 
      University, Xi'an 710032, China.
FAU - Shuai, Yi
AU  - Shuai Y
AD  - Department of Oral Histology and Pathology, School of Stomatology, Fourth 
      Military Medical University, Xi'an 710032, China.
FAU - Liao, Li
AU  - Liao L
AD  - Department of Oral Histology and Pathology, School of Stomatology, Fourth 
      Military Medical University, Xi'an 710032, China.
FAU - Jin, Yan
AU  - Jin Y
AD  - Department of Oral Histology and Pathology, School of Stomatology, Fourth 
      Military Medical University, Xi'an 710032, China.
FAU - Yang, Deqin
AU  - Yang D
AD  - Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical 
      University; Chongqing Key Laboratory of Oral Diseases and Biomedical Science, 
      Chongqing 400015, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Estrogens)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Bone Marrow Cells/cytology
MH  - *Cell Movement
MH  - Chemokine CCL2/*metabolism
MH  - Estrogens/*deficiency
MH  - Female
MH  - Gene Expression Regulation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteoporosis/immunology/*metabolism
MH  - Ovariectomy
MH  - T-Lymphocytes/*cytology
EDAT- 2014/01/11 06:00
MHDA- 2014/07/12 06:00
CRDT- 2014/01/11 06:00
PHST- 2014/01/11 06:00 [entrez]
PHST- 2014/01/11 06:00 [pubmed]
PHST- 2014/07/12 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Jan;30(1):19-22.