PMID- 24406030
OWN - NLM
STAT- MEDLINE
DCOM- 20140911
LR  - 20211021
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 4
IP  - 5
DP  - 2013 Oct 13
TI  - Insult-dependent effect of bone marrow cell therapy on inflammatory response in a 
      murine model of extrapulmonary acute respiratory distress syndrome.
PG  - 123
LID - 10.1186/scrt334 [doi]
AB  - INTRODUCTION: Administration of bone marrow-derived cells produces beneficial 
      effects in experimental extrapulmonary acute respiratory distress syndrome 
      (ARDS). However, there are controversies regarding the effects of timing of cell 
      administration and initial insult severity on inflammatory response. We evaluated 
      the effects of bone marrow-derived mononuclear cells (BMDMC) in two models of 
      extrapulmonary ARDS once lung morphofunctional changes had already been 
      installed. METHODS: BALB/c mice received lipopolysaccharide (LPS) 
      intraperitoneally (5 mg/kg in 0.5 ml saline) or underwent cecal ligation and 
      puncture (CLP). Control mice received saline intraperitoneally (0.5 ml) or 
      underwent sham surgery. At 24 hours, groups were further randomized to receive 
      saline or BMDMC (2 x 10(6)) intravenously. Lung mechanics, histology, and humoral 
      and cellular parameters of lung inflammation and remodeling were analyzed 1, 3 
      and 7 days after ARDS induction. RESULTS: BMDMC therapy led to improved survival 
      in the CLP group, reduced lung elastance, alveolar collapse, tissue and 
      bronchoalveolar lavage fluid cellularity, collagen fiber content, and 
      interleukin-1beta and increased chemokine (keratinocyte-derived chemokine and 
      monocyte chemotactic protein-1) expression in lung tissue regardless of the 
      experimental ARDS model. Intercellular adhesion molecule-1 and vascular cell 
      adhesion molecule-1 expression in lung tissue increased after cell therapy 
      depending on the insult (LPS or CLP). CONCLUSIONS: BMDMC therapy at day 1 
      successfully reduced lung inflammation and remodeling, thus contributing to 
      improvement of lung mechanics in both extrapulmonary ARDS models. Nevertheless, 
      the different inflammatory responses induced by LPS and CLP resulted in distinct 
      effects of BMDMC therapy. These data may be useful in the clinical setting, as 
      they suggest that the type of initial insult plays a key role in the outcome of 
      treatment.
FAU - Maron-Gutierrez, Tatiana
AU  - Maron-Gutierrez T
FAU - Silva, Johnatas Dutra
AU  - Silva JD
FAU - Cruz, Fernanda Ferreira
AU  - Cruz FF
FAU - Alegria, Samantha
AU  - Alegria S
FAU - Xisto, Debora Goncalves
AU  - Xisto DG
FAU - Assis, Edson Fernandes
AU  - Assis EF
FAU - Castro-Faria-Neto, Hugo Caire
AU  - Castro-Faria-Neto HC
FAU - Dos Santos, Claudia Chimisso
AU  - Dos Santos CC
FAU - Morales, Marcelo Marcos
AU  - Morales MM
FAU - Rocco, Patricia Rieken Macedo
AU  - Rocco PR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131013
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-1beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 147037-79-4 (keratinocyte-derived chemokines)
SB  - IM
CIN - Stem Cell Res Ther. 2013;4(6):143. PMID: 24279925
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - *Cell- and Tissue-Based Therapy
MH  - Chemokine CCL2/genetics/metabolism
MH  - Chemokines/genetics/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Inflammation Mediators/*metabolism
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Interleukin-1beta/genetics/metabolism
MH  - Leukocytes, Mononuclear/cytology/transplantation
MH  - Lung/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - RNA, Messenger/metabolism
MH  - Respiratory Distress Syndrome/immunology/pathology/therapy
MH  - Time Factors
MH  - Vascular Cell Adhesion Molecule-1/genetics/metabolism
PMC - PMC3856598
EDAT- 2014/01/11 06:00
MHDA- 2014/09/12 06:00
PMCR- 2013/10/13
CRDT- 2014/01/11 06:00
PHST- 2013/05/14 00:00 [received]
PHST- 2013/10/03 00:00 [accepted]
PHST- 2014/01/11 06:00 [entrez]
PHST- 2014/01/11 06:00 [pubmed]
PHST- 2014/09/12 06:00 [medline]
PHST- 2013/10/13 00:00 [pmc-release]
AID - scrt334 [pii]
AID - 10.1186/scrt334 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2013 Oct 13;4(5):123. doi: 10.1186/scrt334.