PMID- 24407181
OWN - NLM
STAT- MEDLINE
DCOM- 20141114
LR  - 20211021
IS  - 1745-7262 (Electronic)
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 16
IP  - 2
DP  - 2014 Mar-Apr
TI  - The biochemical effects of ischemia-reperfusion injury in the ipsilateral and 
      contralateral testes of rats and the protective role of melatonin.
PG  - 314-8
LID - 10.4103/1008-682X.122202 [doi]
AB  - Testicular torsion (TT) is a serious urologic emergency that is observed in 
      adolescent males and that can lead to infertility if left untreated. The 
      ischemia-reperfusion (I/R) injury due to TT has been implicated in the 
      pathogenesis of testicular damage. We investigated the effects of melatonin on 
      oxidative damage in the ipsilateral and contralateral testes of rats induced by 
      unilateral TT. A total of 21 prepubertal male Wistar albino rats were divided 
      into three groups, each consisting of seven rats. In Group 1 (SHAM group): a sham 
      operation to the left testis and bilateral orchiectomy were performed. In Group 2 
      (I/R group): I/R injury was created by rotating the left testis 720 degrees  in a 
      clockwise direction for 2 h and detorsing the testis after 2 h. Group 3 (I/R + 
      MEL group): rats were subjected to I/R injury and one-shot melatonin injection 
      (50 mg kg?1, intraperitoneal (i.p.)). The testes of the rats were excised 
      bilaterally in all groups. The testicular tissue activities of antioxidant 
      catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase enzymes 
      (GSH-Px), and the tissue levels of malondialdehyde (MDA), protein carbonyl (PC) 
      and nitric oxide (NO) were determined. Administration of melatonin caused a 
      significant decrease in lipid peroxidation and enzyme activities in the 
      ipsilateral testis when compared with the control group (P < 0.05). All of the 
      changes in the enzyme activities of the contralateral testis were insignificant 
      (P > 0.05). MDA levels were signifi cantly altered in the contralateral testis (P 
      = 0.009). Melatonin administration decreased the deleterious effects of I/R 
      injury in the ipsilateral torted testes of the rats. The contralateral testes 
      were slightly affected by unilateral TT.
FAU - Parlaktas, Bekir S
AU  - Parlaktas BS
AD  - Department of Urology, Gaziosmanpasa University, Faculty of Medicine, Tokat, 
      Turkey.
FAU - Atilgan, Dogan
AU  - Atilgan D
FAU - Ozyurt, Huseyin
AU  - Ozyurt H
FAU - Gencten, Yusuf
AU  - Gencten Y
FAU - Akbas, Ali
AU  - Akbas A
FAU - Erdemir, Fikret
AU  - Erdemir F
FAU - Uluocak, Nihat
AU  - Uluocak N
LA  - eng
PT  - Journal Article
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Catalase/metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Melatonin/*pharmacology
MH  - Nitric Oxide/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*metabolism/prevention & control
MH  - Superoxide Dismutase/metabolism
MH  - Testis/drug effects/*pathology
PMC - PMC3955347
EDAT- 2014/01/11 06:00
MHDA- 2014/11/15 06:00
PMCR- 2014/03/01
CRDT- 2014/01/11 06:00
PHST- 2014/01/11 06:00 [entrez]
PHST- 2014/01/11 06:00 [pubmed]
PHST- 2014/11/15 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - 122202 [pii]
AID - AJA-16-314 [pii]
AID - 10.4103/1008-682X.122202 [doi]
PST - ppublish
SO  - Asian J Androl. 2014 Mar-Apr;16(2):314-8. doi: 10.4103/1008-682X.122202.