PMID- 24407591
OWN - NLM
STAT- MEDLINE
DCOM- 20140424
LR  - 20211203
IS  - 1522-1547 (Electronic)
IS  - 0193-1857 (Print)
IS  - 0193-1857 (Linking)
VI  - 306
IP  - 5
DP  - 2014 Mar 1
TI  - Rab1a regulates sorting of early endocytic vesicles.
PG  - G412-24
LID - 10.1152/ajpgi.00118.2013 [doi]
AB  - We previously reported that Rab1a is associated with asialoorosomucoid 
      (ASOR)-containing early endocytic vesicles, where it is required for their 
      microtubule-based motility. In Rab1a knockdown (KD) cell lines, ASOR failed to 
      segregate from its receptor and, consequently, did not reach lysosomes for 
      degradation, indicating a defect in early endosome sorting. Although Rab1 is 
      required for Golgi/endoplasmic reticulum trafficking, this process was 
      unaffected, likely due to retained expression of Rab1b in these cells. The 
      present study shows that Rab1a has a more general role in endocytic vesicle 
      processing that extends to EGF and transferrin (Tfn) trafficking. Compared with 
      results in control Huh7 cells, EGF accumulated in aggregates within Rab1a KD 
      cells, failing to reach lysosomal compartments. Tfn, a prototypical example of 
      recycling cargo, accumulated in a Rab11-mediated slow-recycling compartment in 
      Rab1a KD cells, in contrast to control cells, which sort Tfn into a 
      fast-recycling Rab4 compartment. These data indicate that Rab1a is an important 
      regulator of early endosome sorting for multiple cargo species. The effectors and 
      accessory proteins recruited by Rab1a to early endocytic vesicles include the 
      minus-end-directed kinesin motor KifC1, while others remain to be discovered.
FAU - Mukhopadhyay, Aparna
AU  - Mukhopadhyay A
AD  - Department of Anatomy and Structural Biology, Albert Einstein College of 
      Medicine, Bronx, New York;
FAU - Quiroz, Jose A
AU  - Quiroz JA
FAU - Wolkoff, Allan W
AU  - Wolkoff AW
LA  - eng
GR  - DK-098408/DK/NIDDK NIH HHS/United States
GR  - P30 DK041296/DK/NIDDK NIH HHS/United States
GR  - T34 GM008718/GM/NIGMS NIH HHS/United States
GR  - DK-23026/DK/NIDDK NIH HHS/United States
GR  - DK-41908/DK/NIDDK NIH HHS/United States
GR  - DK-41296/DK/NIDDK NIH HHS/United States
GR  - R01 DK098408/DK/NIDDK NIH HHS/United States
GR  - T32 GM007288/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140109
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (Asialoglycoproteins)
RN  - 0 (KIFC1 protein, human)
RN  - 0 (asialoovomucoid)
RN  - 37281-36-0 (Ovomucin)
RN  - EC 3.6.4.4 (Kinesins)
RN  - EC 3.6.5.2 (rab1 GTP-Binding Proteins)
SB  - IM
MH  - Asialoglycoproteins/metabolism
MH  - Biological Transport
MH  - Cell Line, Tumor
MH  - Endocytosis
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation/*physiology
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Kinesins/genetics/metabolism
MH  - Ovomucin/metabolism
MH  - Transport Vesicles/*physiology
MH  - rab1 GTP-Binding Proteins/genetics/*metabolism
PMC - PMC3949023
OTO - NOTNLM
OT  - EGF
OT  - Rab1a
OT  - endocytosis
OT  - sorting
OT  - transferrin
EDAT- 2014/01/11 06:00
MHDA- 2014/04/25 06:00
PMCR- 2015/03/01
CRDT- 2014/01/11 06:00
PHST- 2014/01/11 06:00 [entrez]
PHST- 2014/01/11 06:00 [pubmed]
PHST- 2014/04/25 06:00 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
AID - ajpgi.00118.2013 [pii]
AID - GI-00118-2013 [pii]
AID - 10.1152/ajpgi.00118.2013 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2014 Mar 1;306(5):G412-24. doi: 
      10.1152/ajpgi.00118.2013. Epub 2014 Jan 9.