PMID- 24409809
OWN - NLM
STAT- MEDLINE
DCOM- 20141020
LR  - 20140305
IS  - 1607-8438 (Electronic)
IS  - 0300-8207 (Linking)
VI  - 55
IP  - 2
DP  - 2014 Apr
TI  - Glutathione protects human nucleus pulposus cells from cell apoptosis and 
      inhibition of matrix synthesis.
PG  - 132-9
LID - 10.3109/03008207.2013.876421 [doi]
AB  - Abstract Cell death (apoptosis and necrosis) and extracellular matrix destruction 
      induced by oxidative stress have been suggested to be closely involved in the 
      process of disc degeneration. Glutathione, a natural peptide as a powerful 
      antioxidant in human cytoplasm, plays an important role in protecting living 
      cells. This study is to investigate whether glutathione could retard degenerated 
      phenotypes in cultured disc cells. Human nucleus pulposus cells were isolated and 
      cultured in alginate beads and subsequently treated with a pro-oxidant H2O2 alone 
      or a pro-inflammatory cytokine IL-1beta alone or either of them together with 
      glutathione. It was shown that H2O2 dose-dependently promoted nucleus pulposus 
      cell apoptosis detected by terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL) staining and decreased mRNA levels of matrix proteins 
      aggrecan and type II collagen determined by quantitative reverse 
      transcription-polymerase chain reaction (RT-PCR). IL-1beta could induce production 
      of nitric oxide and decrease of proteoglycan, detected by the Griess reagent and 
      the dimethyl methylene blue, respectively. The deleterious effects of either H2O2 
      or IL-1beta could be efficiently prevented by glutathione. These results indicated 
      that glutathione might be considered as an option for intervention of disc 
      degeneration.
FAU - Yang, Dazhi
AU  - Yang D
AD  - Department of Spinal Surgery, The Second Affiliated Hospital of Jinan University 
      Medical School , Shenzhen , China and.
FAU - Wang, Daidong
AU  - Wang D
FAU - Shimer, Adam
AU  - Shimer A
FAU - Shen, Francis H
AU  - Shen FH
FAU - Li, Xudong
AU  - Li X
FAU - Yang, Xinlin
AU  - Yang X
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140124
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (Collagen Type II)
RN  - 0 (IL1B protein, human)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Oxidants)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cells, Cultured
MH  - Child
MH  - Collagen Type II/*biosynthesis
MH  - Extracellular Matrix/*metabolism/pathology
MH  - Female
MH  - Glutathione/*pharmacology
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Interleukin-1beta/pharmacology
MH  - Intervertebral Disc/*metabolism/pathology
MH  - Intervertebral Disc Degeneration/metabolism/pathology
MH  - Male
MH  - Oxidants/pharmacology
EDAT- 2014/01/15 06:00
MHDA- 2014/10/21 06:00
CRDT- 2014/01/14 06:00
PHST- 2014/01/14 06:00 [entrez]
PHST- 2014/01/15 06:00 [pubmed]
PHST- 2014/10/21 06:00 [medline]
AID - 10.3109/03008207.2013.876421 [doi]
PST - ppublish
SO  - Connect Tissue Res. 2014 Apr;55(2):132-9. doi: 10.3109/03008207.2013.876421. Epub 
      2014 Jan 24.