PMID- 24410800
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20211021
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 45
IP  - 1
DP  - 2014 Jan 10
TI  - Genotyping of a microsatellite locus to differentiate clinical Ostreid 
      herpesvirus 1 specimens.
PG  - 3
LID - 10.1186/1297-9716-45-3 [doi]
AB  - Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the 
      Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been 
      associated with mortality outbreaks in different bivalve species including the 
      Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events 
      have been reported among C. gigas in Europe in relation to the detection of a 
      variant of OsHV-1, called muVar. Since 2009, this variant has been mainly detected 
      in France. These results raise questions about the emergence and the virulence of 
      this variant. The search for association between specific virus genetic markers 
      and clinical symptoms is of great interest and the characterization of the 
      genetic variability of OsHV-1 specimens is an area of growing interest. 
      Determination of nucleotide sequences of PCR-amplified virus DNA fragments has 
      already been used to characterize OsHV-1 specimens and virus variants have thus 
      been described. However, the virus DNA sequencing approach is time-consuming in 
      the high-scale format. Identification and genotyping of highly polymorphic 
      microsatellite loci appear as a suitable approach. The main objective of the 
      present study was the development of a genotyping method in order to characterise 
      clinical OsHV-1 specimens by targeting a particular microsatellite locus located 
      in the ORF4 area. Genotyping results were compared to sequences already 
      available. An excellent correlation was found between the detected genotypes and 
      the corresponding sequences showing that the genotyping approach allowed an 
      accurate discrimination between virus specimens.
FAU - Renault, Tristan
AU  - Renault T
AD  - Ifremer, Unite Sante, Genetique et Microbiologie des Mollusques, Laboratoire de 
      Genetique et Pathologie des Mollusques Marins, 17390 La Tremblade, France. 
      trenault@ifremer.fr.
FAU - Tchaleu, Gwenaelle
AU  - Tchaleu G
FAU - Faury, Nicole
AU  - Faury N
FAU - Moreau, Pierrick
AU  - Moreau P
FAU - Segarra, Amelie
AU  - Segarra A
FAU - Barbosa-Solomieu, Valerie
AU  - Barbosa-Solomieu V
FAU - Lapegue, Sylvie
AU  - Lapegue S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140110
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Crassostrea/*virology
MH  - DNA Viruses/*genetics/metabolism
MH  - DNA, Viral/*genetics/metabolism
MH  - France
MH  - Genotyping Techniques/*methods/veterinary
MH  - Japan
MH  - *Microsatellite Repeats
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Sequence Alignment/veterinary
MH  - United States
PMC - PMC3897894
EDAT- 2014/01/15 06:00
MHDA- 2014/09/16 06:00
PMCR- 2014/01/01
CRDT- 2014/01/14 06:00
PHST- 2013/04/14 00:00 [received]
PHST- 2013/12/19 00:00 [accepted]
PHST- 2014/01/14 06:00 [entrez]
PHST- 2014/01/15 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 1297-9716-45-3 [pii]
AID - 10.1186/1297-9716-45-3 [doi]
PST - epublish
SO  - Vet Res. 2014 Jan 10;45(1):3. doi: 10.1186/1297-9716-45-3.