PMID- 24411654
OWN - NLM
STAT- MEDLINE
DCOM- 20141124
LR  - 20221207
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 96
IP  - 2
DP  - 2014 Apr
TI  - Antiviral activity and underlying molecular mechanisms of Matrine against porcine 
      reproductive and respiratory syndrome virus in vitro.
PG  - 323-7
LID - S0034-5288(13)00391-3 [pii]
LID - 10.1016/j.rvsc.2013.12.009 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS), caused by porcine 
      reproductive and respiratory syndrome virus (PRRSV), is an acute infectious 
      disease. The prevalence of PRRS has made swine industry suffered huge financial 
      losses. Matrine, a natural compound, has been demonstrated to possess anti-PRRSV 
      activity in Marc-145 cells. However, the underlying molecular mechanisms were 
      still unknown. The main objective of our study was to discuss the effect of 
      Matrine on PRRSV N protein expression and PRRSV induced apoptosis. Indirect 
      immunofluorescence assay (IFA) and Western blot were used to assess the effect of 
      Matrine on N protein expression. Apoptosis was analyzed by fluorescence staining. 
      In addition, the effect of Matrine on caspase-3 activation was investigated by 
      Western blot. Indirect immunofluorescence assay and Western blot analysis 
      demonstrated that Matrine could inhibit N protein expression in Marc-145 cells. 
      And Matrine was found to be able to impair PRRSV-induced apoptosis by inhibiting 
      caspase-3 activation.
CI  - Copyright (c) 2013. Published by Elsevier Ltd.
FAU - Sun, Na
AU  - Sun N
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China.
FAU - Wang, Zhi-Wei
AU  - Wang ZW
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China.
FAU - Wu, Cai-Hong
AU  - Wu CH
AD  - Jiangsu Animal Husbandry & Veterinary College, Taizhou, Jiangsu 225300, PR China.
FAU - Li, E
AU  - Li E
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China.
FAU - He, Jun-Ping
AU  - He JP
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China.
FAU - Wang, Shao-Yu
AU  - Wang SY
AD  - University of Western Sydney, School of Medicine, Locked Bag 1797, Penrith 2751, 
      NSW, Australia.
FAU - Hu, Yuan-Liang
AU  - Hu YL
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
FAU - Lei, Hai-Min
AU  - Lei HM
AD  - School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 
      100102, PR China.
FAU - Li, Hong-Quan
AU  - Li HQ
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China. Electronic address: livets@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131219
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Alkaloids)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Quinolizines)
RN  - 0 (Matrines)
SB  - IM
MH  - Alkaloids/*pharmacology/therapeutic use
MH  - Animals
MH  - Apoptosis/drug effects/*immunology
MH  - Blotting, Western/veterinary
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Microscopy, Fluorescence/veterinary
MH  - Nucleocapsid Proteins/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*drug therapy/immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Quinolizines/*pharmacology/therapeutic use
MH  - Swine
MH  - Matrines
OTO - NOTNLM
OT  - Antiviral
OT  - Apoptosis
OT  - Matrine
OT  - N protein
OT  - PRRSV
EDAT- 2014/01/15 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/01/14 06:00
PHST- 2013/09/12 00:00 [received]
PHST- 2013/12/13 00:00 [revised]
PHST- 2013/12/15 00:00 [accepted]
PHST- 2014/01/14 06:00 [entrez]
PHST- 2014/01/15 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0034-5288(13)00391-3 [pii]
AID - 10.1016/j.rvsc.2013.12.009 [doi]
PST - ppublish
SO  - Res Vet Sci. 2014 Apr;96(2):323-7. doi: 10.1016/j.rvsc.2013.12.009. Epub 2013 Dec 
      19.