PMID- 24412152
OWN - NLM
STAT- MEDLINE
DCOM- 20141124
LR  - 20201209
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 65
DP  - 2014 Apr
TI  - Interferon regulatory factor-1 (IRF-1) interacts with regulated in development 
      and DNA damage response 2 (REDD2) in the cytoplasm of mouse bone marrow cells.
PG  - 41-50
LID - S0141-8130(14)00006-3 [pii]
LID - 10.1016/j.ijbiomac.2014.01.005 [doi]
AB  - IRF-1 is a critical hematopoietic transcription factor, which regulates cell 
      growth, development of immune cells, immune response, tumor suppression, 
      apoptosis and autophagy in mammalian cells. Protein-protein interactions of IRF-1 
      in mouse bone marrow cells (BMCs) by GST-IRF-1 pull-down followed by mass 
      spectrometry, coimmunoprecipitation, immunoblotting and colocalization show that 
      regulated in development and DNA damage response 2 (REDD2) is an 
      IRF-1-interacting protein. REDD2 is a highly conserved mammalian regulatory 
      protein of the TSC2/mTOR pathway. It is structurally similar to REDD1 but has a 
      distinct loop region. Cellular IRF-1 and REDD2 complex is present in the 
      cytoplasm of BMCs as distinct speckles in punctate pattern. In vitro interaction 
      of recombinant IRF-1 and REDD2 shows their physical interaction. Taken together, 
      our results suggest that IRF-1 physically interacts with REDD2 in the large 
      cytoplasmic protein complex, which may function as cellular signaling proteins 
      for 'cross-talk' of mTOR and cytokine pathways during regulation of cell 
      growth/proliferation, apoptosis and autophagy of mammalian bone marrow cells 
      during health and disease.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Gupta, Manish
AU  - Gupta M
AD  - Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru 
      University, New Delhi, 110067, India.
FAU - Rath, Pramod C
AU  - Rath PC
AD  - Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru 
      University, New Delhi, 110067, India. Electronic address: pcrath@mail.jnu.ac.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140109
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Ddit4l protein, mouse)
RN  - 0 (Interferon Regulatory Factor-1)
RN  - 0 (Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Tubulin)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis
MH  - Autophagy
MH  - Bone Marrow Cells/*cytology
MH  - Cell Proliferation
MH  - Cytoplasm/*metabolism
MH  - DNA-Binding Proteins
MH  - Humans
MH  - Immunoprecipitation
MH  - Interferon Regulatory Factor-1/chemistry/*metabolism
MH  - Male
MH  - Mice
MH  - Molecular Docking Simulation
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Proteins/chemistry/*metabolism
MH  - Transcription Factors
MH  - Tubulin/metabolism
OTO - NOTNLM
OT  - Bone marrow cells
OT  - Interferon regulatory factor-1
OT  - Protein-protein interaction
OT  - Regulated in development and DNA damage response 2
EDAT- 2014/01/15 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/01/14 06:00
PHST- 2013/09/14 00:00 [received]
PHST- 2013/12/12 00:00 [revised]
PHST- 2014/01/02 00:00 [accepted]
PHST- 2014/01/14 06:00 [entrez]
PHST- 2014/01/15 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0141-8130(14)00006-3 [pii]
AID - 10.1016/j.ijbiomac.2014.01.005 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2014 Apr;65:41-50. doi: 10.1016/j.ijbiomac.2014.01.005. Epub 
      2014 Jan 9.