PMID- 24412690
OWN - NLM
STAT- MEDLINE
DCOM- 20140926
LR  - 20191210
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 947-948
DP  - 2014 Feb 1
TI  - Optimization of solvent bar microextraction combined with gas chromatography for 
      preconcentration and determination of methadone in human urine and plasma 
      samples.
PG  - 75-82
LID - S1570-0232(13)00698-3 [pii]
LID - 10.1016/j.jchromb.2013.12.011 [doi]
AB  - In this study, solvent bar microextraction combined with gas chromatography-flame 
      ionization detector (GC-FID) was used for preconcentration and determination of 
      methadone in human body fluids. The target drug was extracted from an aqueous 
      sample with pH 11.5 (source phase) into an organic extracting solvent 
      (1-Undecanol) located inside the pores and lumen of a polypropylene hollow fiber 
      as a receiving phase. To obtain high extraction efficiency, the effect of 
      different variables on the extraction efficiency was studied using an 
      experimental design. The variables of interest were the organic phase type, 
      source phase pH, ionic strength, stirring rate, extraction time, concentration of 
      Triton X-100, and extraction temperature, which were first investigated by 
      Plackett-Burman design and subsequently by central composite design (CCD). So 
      that the optimum experimental condition was obtained when the sodium chloride 
      concentration was 5% (w/v); stirring rate, 700 rpm; extraction temperature, 20 
       degrees C; extraction time, 45 min and pH of the aqueous sample, 11.5. Under the 
      optimized conditions, the preconcentration factors were between 275 and 300. The 
      calibration curves were linear in the concentration range of 10-1500 mug L(-1). 
      The limits of detection (LODs) were 2.7-7 and relative standard deviations (RSDs) 
      of the proposed method were 5.9-7.3%. Ultimately, the applicability of the 
      current method was evaluated by the extraction and determination of methadone in 
      different biological samples.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Ebrahimzadeh, Homeira
AU  - Ebrahimzadeh H
AD  - Department of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran. 
      Electronic address: h-ebrahim@sbu.ac.ir.
FAU - Mirbabaei, Fatemeh
AU  - Mirbabaei F
AD  - Department of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
FAU - Asgharinezhad, Ali Akbar
AU  - Asgharinezhad AA
AD  - Department of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
FAU - Shekari, Nafiseh
AU  - Shekari N
AD  - Department of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
FAU - Mollazadeh, Narges
AU  - Mollazadeh N
AD  - Department of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20131217
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Analgesics, Opioid)
RN  - UC6VBE7V1Z (Methadone)
SB  - IM
MH  - Analgesics, Opioid/blood/*isolation & purification/urine
MH  - Chromatography, Gas/methods
MH  - Doping in Sports
MH  - Humans
MH  - Methadone/blood/*isolation & purification/urine
MH  - Solid Phase Microextraction/instrumentation/*methods
OTO - NOTNLM
OT  - Biological sample
OT  - Experimental design
OT  - Methadone
OT  - Preconcentration
OT  - Solvent bar microextraction
EDAT- 2014/01/15 06:00
MHDA- 2014/09/27 06:00
CRDT- 2014/01/14 06:00
PHST- 2013/05/05 00:00 [received]
PHST- 2013/11/25 00:00 [revised]
PHST- 2013/12/08 00:00 [accepted]
PHST- 2014/01/14 06:00 [entrez]
PHST- 2014/01/15 06:00 [pubmed]
PHST- 2014/09/27 06:00 [medline]
AID - S1570-0232(13)00698-3 [pii]
AID - 10.1016/j.jchromb.2013.12.011 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Feb 1;947-948:75-82. doi: 
      10.1016/j.jchromb.2013.12.011. Epub 2013 Dec 17.