PMID- 24414131
OWN - NLM
STAT- MEDLINE
DCOM- 20150623
LR  - 20211021
IS  - 1436-3305 (Electronic)
IS  - 1436-3291 (Print)
IS  - 1436-3291 (Linking)
VI  - 17
IP  - 4
DP  - 2014 Oct
TI  - Importance of formalin fixing conditions for HER2 testing in gastric cancer: 
      immunohistochemical staining and fluorescence in situ hybridization.
PG  - 638-47
LID - 10.1007/s10120-013-0329-8 [doi]
AB  - BACKGROUND: Accurate and reliable assessment of human epidermal growth factor 
      receptor type 2 (HER2) status is important for selecting patients with gastric 
      cancer who may benefit from trastuzumab treatment. Here we examined the impact of 
      formalin fixing conditions on HER2 immunohistochemistry (IHC) and fluorescence in 
      situ hybridization (FISH) in xenografted tumor tissues. METHODS: Xenografted 
      tumor tissues of the human gastric cancer cell lines NCI-N87, SCH, and SNU-16 
      were collected and kept at room temperature for 0, 6, or 24 h before being fixed 
      with 10 % neutral buffered formalin (NBF) for 24 h or 5, 7, or 10 days and 
      embedded in paraffin. Use of 10 % NBF, 20 % NBF, or nonbuffered formalin as 
      fixative was investigated. RESULTS: The HER2 IHC scores for NCI-N87, SCH, and 
      SNU-16 tumors were 3+, 2+, and 1+, respectively, when specimens were fixed with 
      10 % NBF for 24 h immediately after resection of the tumors. Specimens left for 
      longer than 6 h before fixation had shrinkage of the tumor periphery and 
      decreased immunostaining intensity in this region in all specimens. In SCH and 
      SNU-16 specimens, starting fixation 24 h after tumor tissue collection induced 
      autolysis and reduction of the number of stained cells, and 10-day-fixation 
      lowered the HER2 score. Prolongation of fixation time did not affect FISH 
      results, but if samples were left for more than 6 h before fixation, the FISH 
      score was strongly reduced in SCH specimens (2.3 to 1.3). Reduced IHC staining 
      intensity was observed with 20 % NBF and nonbuffered formalin compared to 10 % 
      NBF. CONCLUSIONS: The time to and length of fixation of tumor specimens can 
      affect HER2 IHC and FISH scores. The fixative used can affect IHC results.
FAU - Yamashita-Kashima, Yoriko
AU  - Yamashita-Kashima Y
AD  - Product Research Department, Chugai Pharmaceutical Co., Ltd, 200, Kajiwara, 
      Kamakura, Kanagawa, 247-8530, Japan.
FAU - Shu, Sei
AU  - Shu S
FAU - Yorozu, Keigo
AU  - Yorozu K
FAU - Hashizume, Kaoru
AU  - Hashizume K
FAU - Moriya, Yoichiro
AU  - Moriya Y
FAU - Fujimoto-Ouchi, Kaori
AU  - Fujimoto-Ouchi K
FAU - Harada, Naoki
AU  - Harada N
LA  - eng
PT  - Journal Article
DEP - 20140112
PL  - Japan
TA  - Gastric Cancer
JT  - Gastric cancer : official journal of the International Gastric Cancer Association 
      and the Japanese Gastric Cancer Association
JID - 100886238
RN  - 0 (Biomarkers, Tumor)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
EIN - Gastric Cancer. 2014 Oct;17(4):648
MH  - Animals
MH  - Biomarkers, Tumor/analysis/metabolism
MH  - Breast Neoplasms/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Female
MH  - *Formaldehyde
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Receptor, ErbB-2/*analysis/metabolism
MH  - Stomach Neoplasms/metabolism/*pathology
MH  - Time Factors
MH  - Tissue Fixation/*methods
MH  - Xenograft Model Antitumor Assays
PMC - PMC4169649
EDAT- 2014/01/15 06:00
MHDA- 2015/06/24 06:00
PMCR- 2014/01/12
CRDT- 2014/01/14 06:00
PHST- 2013/05/21 00:00 [received]
PHST- 2013/12/16 00:00 [accepted]
PHST- 2014/01/14 06:00 [entrez]
PHST- 2014/01/15 06:00 [pubmed]
PHST- 2015/06/24 06:00 [medline]
PHST- 2014/01/12 00:00 [pmc-release]
AID - 329 [pii]
AID - 10.1007/s10120-013-0329-8 [doi]
PST - ppublish
SO  - Gastric Cancer. 2014 Oct;17(4):638-47. doi: 10.1007/s10120-013-0329-8. Epub 2014 
      Jan 12.