PMID- 2441419
OWN - NLM
STAT- MEDLINE
DCOM- 19870828
LR  - 20190712
IS  - 0091-3057 (Print)
IS  - 0091-3057 (Linking)
VI  - 27
IP  - 1
DP  - 1987 May
TI  - Dietary fat source influences neuronal mitochondrial monoamine oxidase activity 
      and macronutrient selection in rats.
PG  - 1-6
AB  - We previously reported that qualitative changes in dietary fat influence certain 
      monoaminergic mediated behaviours such as pain sensitivity and thermoregulation 
      in a cold environment after an amphetamine challenge. The purpose of this study 
      was to further explore the behavioural consequences of alterations in dietary fat 
      intake by examining another behaviour known to be mediated by the 
      monoamines--food intake regulation--and to begin investigating a biochemical link 
      between dietary fat composition and behaviour. Rats were stabilized to 20% (w/w) 
      soybean oil (SBO) or lard diets for 10 days and then allowed to select for 
      protein (PRO) and carbohydrate (CHO) intake. While total food intake was 
      unchanged, rats fed the SBO diet selected lower PRO (3.1 +/- 0.6 vs. 4.9 +/- 0.6 
      g/day, SBO vs. lard, respectively) and higher CHO (9.6 +/- 0.7 vs. 7.8 +/- 1.2) 
      intakes than those consuming the lard based diet. Comparable differences were 
      seen in a second trial. Current evidence suggests that the regulation of PRO and 
      CHO intake is under serotonergic control. Therefore to determine whether dietary 
      fat is mediating its effect on macronutrient selection via alterations in 
      serotonin (5HT) metabolism, brain stem concentrations of 5HT and its metabolite 
      5-hydroxyindole acetic acid (5HIAA) and whole brain (minus brain stem) 
      mitochondrial monoamine oxidase (MAO) activity were measured in a separate set of 
      animals fed the SBO or lard diets for 28 days. Vmax of MAO was decreased in rats 
      fed the SBO diets (20.2 +/- 7.4 vs. 27.9 +/- 8.9 nmol/mg prot/20') compared to 
      those fed the lard diets. Km was unaltered by dietary fat fed. The change in 
      activity of MAO was insufficient to alter steady-state levels of 5HT or 5HIAA. We 
      propose that changes in neuronal functioning, induced by altered dietary fat, 
      contributed to the differences seen in PRO and CHO selection.
FAU - Crane, S B
AU  - Crane SB
FAU - Greenwood, C E
AU  - Greenwood CE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pharmacol Biochem Behav
JT  - Pharmacology, biochemistry, and behavior
JID - 0367050
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Fats)
RN  - 0 (Dietary Proteins)
RN  - 333DO1RDJY (Serotonin)
RN  - 54-16-0 (Hydroxyindoleacetic Acid)
RN  - EC 1.4.3.4 (Monoamine Oxidase)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/enzymology
MH  - Dietary Carbohydrates
MH  - Dietary Fats/*pharmacology
MH  - Dietary Proteins
MH  - Food Preferences/*drug effects
MH  - Hydroxyindoleacetic Acid/metabolism
MH  - Kinetics
MH  - Male
MH  - Mitochondria/*drug effects/enzymology
MH  - Monoamine Oxidase/*metabolism
MH  - Neurons/drug effects/enzymology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Serotonin/metabolism
EDAT- 1987/05/01 00:00
MHDA- 1987/05/01 00:01
CRDT- 1987/05/01 00:00
PHST- 1987/05/01 00:00 [pubmed]
PHST- 1987/05/01 00:01 [medline]
PHST- 1987/05/01 00:00 [entrez]
AID - 0091-3057(87)90468-0 [pii]
AID - 10.1016/0091-3057(87)90468-0 [doi]
PST - ppublish
SO  - Pharmacol Biochem Behav. 1987 May;27(1):1-6. doi: 10.1016/0091-3057(87)90468-0.