PMID- 24416034
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140113
LR  - 20220129
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 4
DP  - 2013
TI  - Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage 
      marker of the human peripheral blood antigen-presenting cell compartment.
PG  - 495
LID - 10.3389/fimmu.2013.00495 [doi]
LID - 495
AB  - Dendritic cells (DCs) and monocytes are critical regulators and effectors of 
      innate and adaptive immune responses. Monocyte expansion has been described in 
      many pathological states while monocyte and DC deficiency syndromes are 
      relatively recent additions to the catalog of human primary immunodeficiency 
      disorders. Clinically applicable screening tests to diagnose and monitor these 
      conditions are lacking. Conventional strategies for identifying human DCs and 
      monocytes have been based on the use of a lineage gate to exclude lymphocytes, 
      thus preventing simultaneous detection of DCs, monocytes, and lymphocyte subsets. 
      Here we demonstrate that CD4 is a reliable lineage marker for the human 
      peripheral blood antigen-presenting cell compartment that can be used to identify 
      DCs and monocytes in parallel with lymphocytes. Based on this principle, simple 
      modification of a standard lymphocyte phenotyping assay permits simultaneous 
      enumeration of four lymphocyte and five DC/monocyte populations from a single 
      sample. This approach is applicable to clinical samples and facilitates the 
      diagnosis of DC and monocyte disorders in a wide range of clinical settings, 
      including genetic deficiency, neoplasia, and inflammation.
FAU - Jardine, Laura
AU  - Jardine L
AD  - Human Dendritic Cell Laboratory, Institute of Cellular Medicine, Newcastle 
      University , Newcastle upon Tyne , UK.
FAU - Barge, Dawn
AU  - Barge D
AD  - Clinical Immunology, Newcastle upon Tyne Hospitals NHS Foundation Trust , 
      Newcastle upon Tyne , UK.
FAU - Ames-Draycott, Ashley
AU  - Ames-Draycott A
AD  - Human Dendritic Cell Laboratory, Institute of Cellular Medicine, Newcastle 
      University , Newcastle upon Tyne , UK.
FAU - Pagan, Sarah
AU  - Pagan S
AD  - Human Dendritic Cell Laboratory, Institute of Cellular Medicine, Newcastle 
      University , Newcastle upon Tyne , UK.
FAU - Cookson, Sharon
AU  - Cookson S
AD  - Human Dendritic Cell Laboratory, Institute of Cellular Medicine, Newcastle 
      University , Newcastle upon Tyne , UK.
FAU - Spickett, Gavin
AU  - Spickett G
AD  - Clinical Immunology, Newcastle upon Tyne Hospitals NHS Foundation Trust , 
      Newcastle upon Tyne , UK.
FAU - Haniffa, Muzlifah
AU  - Haniffa M
AD  - Human Dendritic Cell Laboratory, Institute of Cellular Medicine, Newcastle 
      University , Newcastle upon Tyne , UK.
FAU - Collin, Matthew
AU  - Collin M
AD  - Human Dendritic Cell Laboratory, Institute of Cellular Medicine, Newcastle 
      University , Newcastle upon Tyne , UK.
FAU - Bigley, Venetia
AU  - Bigley V
AD  - Human Dendritic Cell Laboratory, Institute of Cellular Medicine, Newcastle 
      University , Newcastle upon Tyne , UK.
LA  - eng
GR  - G0800358/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20131227
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
PMC - PMC3873601
OTO - NOTNLM
OT  - dendritic cells
OT  - flow cytometry
OT  - humans
OT  - immunodeficiency
OT  - monocytes
EDAT- 2014/01/15 06:00
MHDA- 2014/01/15 06:01
PMCR- 2013/01/01
CRDT- 2014/01/14 06:00
PHST- 2013/10/01 00:00 [received]
PHST- 2013/12/17 00:00 [accepted]
PHST- 2014/01/14 06:00 [entrez]
PHST- 2014/01/15 06:00 [pubmed]
PHST- 2014/01/15 06:01 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2013.00495 [doi]
PST - epublish
SO  - Front Immunol. 2013 Dec 27;4:495. doi: 10.3389/fimmu.2013.00495. eCollection 
      2013.