PMID- 24416161
OWN - NLM
STAT- MEDLINE
DCOM- 20140908
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 1
DP  - 2014
TI  - Long-term effects of peripubertal binge EtOH exposure on hippocampal microRNA 
      expression in the rat.
PG  - e83166
LID - 10.1371/journal.pone.0083166 [doi]
LID - e83166
AB  - Adolescent binge alcohol abuse induces long-term changes in gene expression, 
      which impacts the physiological stress response and memory formation, two 
      functions mediated in part by the ventral (VH) and dorsal (DH) hippocampus. 
      microRNAs (miRs) are small RNAs that play an important role in gene regulation 
      and are potential mediators of long-term changes in gene expression. Two genes 
      important for regulating hippocampal functions include brain-derived neurotrophic 
      factor (BDNF) and sirtuin-1 (SIRT1), which we identified as putative gene targets 
      of miR-10a-5p, miR-26a, miR-103, miR-495. The purpose of this study was to 
      quantify miR-10a-5p, miR-26a, miR-103, miR-495 expression levels in the dorsal 
      and ventral hippocampus of male Wistar rats during normal pubertal development 
      and then assess the effects of repeated binge-EtOH exposure. In addition, we 
      measured the effects of binge EtOH-exposure on hippocampal Drosha and Dicer mRNA 
      levels, as well as the putative miR target genes, BDNF and SIRT1. Overall, 
      mid/peri-pubertal binge EtOH exposure altered the normal expression patterns of 
      all miRs tested in an age- and brain region-dependent manner and this effect 
      persisted for up to 30 days post-EtOH exposure. Moreover, our data revealed that 
      mid/peri-pubertal binge EtOH exposure significantly affected miR biosynthetic 
      processing enzymes, Drosha and Dicer. Finally, EtOH-induced significant changes 
      in the expression of a subset of miRs, which correlated with changes in the 
      expression of their predicted target genes. Taken together, these data 
      demonstrate that EtOH exposure during pubertal development has long-term effects 
      on miRNA expression in the rat hippocampus.
FAU - Prins, Sarah A
AU  - Prins SA
AD  - Loyola University Chicago Health Science Division, Department of Cell and 
      Molecular Physiology, Maywood, Illinois, United States of America.
FAU - Przybycien-Szymanska, Magdalena M
AU  - Przybycien-Szymanska MM
AD  - Loyola University Chicago Health Science Division, Department of Cell and 
      Molecular Physiology, Maywood, Illinois, United States of America.
FAU - Rao, Yathindar S
AU  - Rao YS
AD  - Loyola University Chicago Health Science Division, Department of Cell and 
      Molecular Physiology, Maywood, Illinois, United States of America.
FAU - Pak, Toni R
AU  - Pak TR
AD  - Loyola University Chicago Health Science Division, Department of Cell and 
      Molecular Physiology, Maywood, Illinois, United States of America.
LA  - eng
GR  - T32 AA013527/AA/NIAAA NIH HHS/United States
GR  - R01 AA021517/AA/NIAAA NIH HHS/United States
GR  - R21AA018398/AA/NIAAA NIH HHS/United States
GR  - R01AA021517/AA/NIAAA NIH HHS/United States
GR  - R21 AA018398/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140108
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 3K9958V90M (Ethanol)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Aging/drug effects/genetics
MH  - Animals
MH  - Binding Sites/genetics
MH  - Binge Drinking/*genetics
MH  - Ethanol/*adverse effects
MH  - *Gene Expression Regulation/drug effects
MH  - Hippocampus/drug effects/growth & development/*metabolism
MH  - Male
MH  - MicroRNAs/biosynthesis/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Sexual Maturation/drug effects/*genetics
MH  - Testosterone/blood
MH  - Time Factors
PMC - PMC3885403
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/01/15 06:00
MHDA- 2014/09/10 06:00
PMCR- 2014/01/08
CRDT- 2014/01/14 06:00
PHST- 2013/09/20 00:00 [received]
PHST- 2013/10/30 00:00 [accepted]
PHST- 2014/01/14 06:00 [entrez]
PHST- 2014/01/15 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
PHST- 2014/01/08 00:00 [pmc-release]
AID - PONE-D-13-38888 [pii]
AID - 10.1371/journal.pone.0083166 [doi]
PST - epublish
SO  - PLoS One. 2014 Jan 8;9(1):e83166. doi: 10.1371/journal.pone.0083166. eCollection 
      2014.